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Cell-free Systems: Recent Advances and Future Outlook

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Abstract

Cell-free systems utilize a subset of cellular components without intact cell wall and/or membranes. The system was first invented for use in fermentation. Subsequent improvements enabled its application in protein synthesis, which is still the most common use of the system. Lately, attempts have been reported where metabolic engineering concepts and techniques were applied to cell-free systems and/or vice-versa. These attempts and advances led to exciting discoveries about biochemical reactions, as well as properties and/or structures of cellular components that make up complex biological systems. This review will first provide a basic overview and brief history of the cell-free system. Then, explanation on recent advances in the field will be provided, followed by notes on the innovative applications. Future outlook of the field will also be covered with the emphasis on how the emerging data science methods can be applied to improve the system and its applicability.

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Acknowledgements

This study was supported by the Hongik University new faculty research support fund.

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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  1. Department of Chemical Engineering, Hongik University, Seoul, 04066, Korea

    Jamin Koo, Jiho Yang & Hyunjun Park

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  1. Jamin Koo
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Koo, J., Yang, J. & Park, H. Cell-free Systems: Recent Advances and Future Outlook. Biotechnol Bioproc E 25, 955–961 (2020). https://doi.org/10.1007/s12257-020-0013-x

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