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AHCC Supplementation Attenuates Muscle Atrophy via Akt Activation in Hindlimb-suspended Rat

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Abstract

We investigated the inhibitory effect of active hexose correlated compound (AHCC) on muscle atrophy in hindlimb-suspended rats. Twenty-four six-week-old male Sprague-Dawley rats were randomly divided into three groups: the control sedentary group (CS, n = 8), the hindlimb-suspended group (HS, n = 8), and the hindlimb-suspended and AHCC-supplemented group (HSA, n = 8). Hindlimb suspension and AHCC supplementation were performed for two weeks. The HSA group was treated with AHCC (1 g/1 kg of body weight (BW)) orally in 0.3 mL of PBS solution, while the HS group received the vehicle (PBS solution) only. After two weeks, the cross-sectional area (CSA) of the HS and HSA groups decreased by approximately 36% (p < 0.05) and 19%, respectively, compared to the CS group. In addition, myonuclear numbers of the HS and HSA groups and the extensor digitorum longus (EDL) muscle weight of the HS group decreased 30% (p < 0.05) and 18%, respectively, compared to the CS group. AHCC supplementation increased the phosphorylation of pAkt/Akt in the HSA group compared to the HS group (p < 0.05). Furthermore, Fbx32 and MuRF1 protein expression in the HSA group recovered to the level of the CS group. Based on these results, AHCC supplementation may have a positive role in the prevention of muscle atrophy via Akt activation in hindlimb-suspended rats.

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Acknowledgments

The authors extend their thanks to the subjects whose participation made this study possible. This work was supported by the Soonchunhyang University Research Fund.

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Authors and Affiliations

  1. Institute of Sports Health Science, Sunmoon University, Asan, 31460, Korea

    Young-Ju Song

  2. Department of physical education, Graduate School of Sunmoon University, Asan, 31460, Korea

    Sang-Hoon Bae & Jin-Young Park

  3. Global Graduate School of Healthcare Business, Soonchunhyang University, Asan, 31538, Korea

    Hye-Jin Lee & My-Young Lee

  4. Department of Medical Science, Soonchunhyang University, Asan, 31538, Korea

    Seung-Jea Lee

  5. Department of Physiology, College of Medicine, Soonchunhyang University, Cheonan, 31151, Korea

    Young-Ki Min & Jeong-Beom Lee

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Correspondence to Jeong-Beom Lee.

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Song, YJ., Bae, SH., Park, JY. et al. AHCC Supplementation Attenuates Muscle Atrophy via Akt Activation in Hindlimb-suspended Rat. Biotechnol Bioproc E 24, 476–482 (2019). https://doi.org/10.1007/s12257-018-0482-3

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  • DOI: https://doi.org/10.1007/s12257-018-0482-3

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