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Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system

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Abstract

Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to screen a high-growth-rate microalga from a mixed culture of microalgal species; Chlorella vulgaris, Chlorella protothecoides, and Chlamydomonas reinhardtii were used as test organisms for our experiments. The time-dependent washout of mixed microalgal pool was executed to successfully enrich the C. reinhardtii, which exhibits the higher growth rate than C. vulgaris and C. protothecoides under turbidostat conditions within 75 h. The domination of C. reinhardtii in the mixed culture was validated by on-line monitoring of growth rate and flowcytometric analysis. For the time-efficient production of microalgal biomass, this screening process has a high potential to segregate the fast-growing microalgal strains from the pool of various uncharacterized microalgal species and random mutants.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Korea

    Won-Sub Shin, Hansol Lee & Min-Gyu Sung

  2. Department of Food and Nutrition, Nambu University, Gwangju, 506-706, Korea

    Kwon-Tack Hwang

  3. Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea

    Simon MoonGeun Jung

  4. Department of Food Science & Technology and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea

    Jong-Hee Kwon

Authors
  1. Won-Sub Shin
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  2. Hansol Lee
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  3. Min-Gyu Sung
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  4. Kwon-Tack Hwang
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  5. Simon MoonGeun Jung
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Correspondence to Simon MoonGeun Jung or Jong-Hee Kwon.

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These authors have contributed equally to this work.

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Shin, WS., Lee, H., Sung, MG. et al. Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system. Biotechnol Bioproc E 21, 268–273 (2016). https://doi.org/10.1007/s12257-015-0716-6

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  • DOI: https://doi.org/10.1007/s12257-015-0716-6

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