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Characterization of Notch Signalling Pathway Members in Normal Prostate, Prostatic Intraepithelial Neoplasia (PIN) and Prostatic Adenocarcinoma

  • Original Article
  • Published:
Pathology & Oncology Research

Abstract

Prostate Cancer (PCa) holds the second place in terms of cancer-related mortality rate among men. The Notch signalling pathway regulates the proliferation and differentiation in embryonic and adult tissues and determines the cell fate. The body of knowledge in the present literature is currently controversial about the effect of the Notch pathway on prostatic cancer. Therefore, the present study aimed to examine the immunolocalization and expression levels of Notch1-4, Jagged1-2, Delta, HES1 and HES5 from among the members of the Notch signalling pathway in tissues of normal, prostatic intraepithelial neoplasia (PIN) and malignant prostate. The current study included a sample of 20 patients with localised prostatic adenocarcinoma, 18 patients with high grade PIN (H-PIN) and 18 normal prostatic tissue. Immunolocalisations of Notch1, 2, 3, 4, Jagged1, 2, Delta, HES1 and HES5 were identified through the immunohistochemical method. The findings of the present study showed that all in-scope members of the Notch signalling pathway were localised in PIN structures to a greater extent than in other tissues and from amongst these members, specifically Notch1, Notch4, Jagged1 and HES1 were at more significant levels. Consequently, the findings of the present study may indicate that the Notch signalling pathway can play a role especially in the formation of PIN structures.

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Abbreviations

ADAM17:

A disintegrin and metalloproteinase domain-containing protein 10 or 17

CSL:

CBF1, Su(H) and LAG-1

DAB:

Diaminobenzidine

DII1:

Delta like 1

HES:

Hairy/Enhancer-of-Split family

HEY:

Hairy/Enhancer-of-Split related with YRPW motif-like protein

NEC :

Notch extracellular domain

NIC :

Notch intracellular domain

N™:

Notch transmembrane domain

PCa:

Prostate cancer

PBS:

phosphate buffered saline

PIN:

Prostatic intraepithelial neoplasia

sc:

Santa Cruz Biotechnology

NB:

Novus Biologicals

BA:

Vector Laboratories

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Acknowledgments

We wish to thank Sibel Ozden Ozer for their technical assistance.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, 07070, Turkey

    Hakan Soylu, Nuray Acar, Ozlem Ozbey & Ismail Ustunel

  2. Department of Pathology, Faculty of Medicine, Akdeniz University, Antalya, 07070, Turkey

    Betul Unal, Ibrahim Bassorgun & Akif Ciftcioglu

  3. Department of Urology, Faculty of Medicine, Akdeniz University, Antalya, 07070, Turkey

    Ismail Turker Koksal

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  1. Hakan Soylu
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  2. Nuray Acar
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Correspondence to Ismail Ustunel.

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Soylu, H., Acar, N., Ozbey, O. et al. Characterization of Notch Signalling Pathway Members in Normal Prostate, Prostatic Intraepithelial Neoplasia (PIN) and Prostatic Adenocarcinoma. Pathol. Oncol. Res. 22, 87–94 (2016). https://doi.org/10.1007/s12253-015-9983-y

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  • DOI: https://doi.org/10.1007/s12253-015-9983-y

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