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Genetic Mapping of Steroidal Glycoalkaloids Using Selective Genotyping in Potato

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Abstract

Steroidal glycoalkaloids (SGAs) are important secondary metabolites in potato which are associated with constitutive host defense mechanism. SGAs like leptines and dehydrocommersonine (DHC) present in wild S. chacoense and S. oplocense, respectively, are known to deter Colorado potato beetle (CPB) feeding. In the current study, LC-MS analysis led to tentative identification of a new SGA with the same molecular mass as α-solanine, solanidenol-chacotriose (SC), which was present in high levels in the CPB susceptible S. tuberosum cv. Shepody, but not in S. oplocense. In a progeny derived from a cross between S. tuberosum, cv. Shepody and S. oplocense derived F1 hybrid, 13213–07, SC was one of the highly variable metabolites along with DHC. Selective genotyping was used for genetic mapping of QTL controlling SC and DHC to chromosome 1. Selective genotyping is dependent on variation between extremes in populations, and it was not effective for QTL mapping of α-solanine and α-chaconine where differences between extremes was low.

Resumen

Los glicoalcaloides esteroidales (SGAs) son metabolitos secundarios importantes en papa, que están asociados con un mecanismo de defensa constitutivo del hospedante. SGAs, como las leptinas y dehidrocomersonina (DHC) presentes en las especies silvestres S. chacoense y S. oplocense, respectivamente, se sabe que desalientan al escarabajo de colorado (CPB) para su alimentación. En el presente estudio, el análisis de LC-MS condujo a la identificación tentativa de un nuevo SGA con la misma masa molecular como α-solanina, solanidenol-chacotriosa (SC), que estaba presente en altos niveles en S. tuberosum cv. Shepody, susceptible al CPB, pero no en S. oplocense. En una progenie derivada de la cruza entre S. tuberosum, cv. Shepody y S. oplocense se derivó un híbrido F1, 13213–07, SC fue uno de los metabolitos altamente variables junto con DHC. Se usó genotipación selectiva para el mapa genético de QTL que controla SC y DHC en el cromosoma 1. La genotipación selectiva depende de la variación entre extremos en poblaciones, y no fue efectiva para el mapeo por QTL de α-solanina y α-chaconina donde la diferencia entre los extremos fue baja.

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Acknowledgements

We thank Leslie Campbell for running MS and collecting metabolomics data, Katheryn Douglass for DNA sequencing, Catherine Clark for providing support for evaluation of CPB defoliation in field conditions, Charlotte Davidson for technical assistance and arrangements in lab, and Woojong Rho and Jaclyn Retallick for collecting leaf samples from 100 potato germplasm lines and helping in DNA extractions.

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  1. Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, 850 Lincoln Road, Fredericton, New Brunswick, E3B 4Z7, Canada

    Jamuna Risal Paudel, Kyle M. Gardner, Benoit Bizimungu, David De Koeyer & Helen H. Tai

  2. Agriculture and Agri-Food Canada, Kentville Research and Development Centre, Kentville, Nova Scotia, Canada

    Jun Song

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  1. Jamuna Risal Paudel
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Supplementary figure S1

Total ion chromatograph for two parents a) S. tuberosum cv Shepody and b) 13213–07. (PNG 84 kb)

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Known genes involved in steroidal gycoalkaloid (SGA) biosynthesis pathway. A) Physical location of SGA biosynthesis genes. Letter ‘a’ and ‘b’ indicate known genes in primary and secondary pathways, respectively, in SGA biosynthesis as shown in B. b) Schematic diagram of SGA biosynthesis pathway with intermediate products and genes involved. (PNG 223 kb)

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Paudel, J.R., Gardner, K.M., Bizimungu, B. et al. Genetic Mapping of Steroidal Glycoalkaloids Using Selective Genotyping in Potato. Am. J. Potato Res. 96, 505–516 (2019). https://doi.org/10.1007/s12230-019-09734-7

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