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Characterization, Pathogenicity and Chemical Control of Streptomyces acidiscabies Associated to Potato Common Scab

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Abstract

The pathogenicity of 10 bacterial isolates was investigated on potato, radish, carrot and beet, including sensitivity and pathogen control efficacy. The isolates were identified by morphological, biochemical and molecular methods. All isolates were pathogenic on radish, carrot, and beet, and were highly virulent on potato. Although the isolates were obtained from different locations in the El Fuerte Valley (Sinaloa, Mexico), they were similar in their morphological, physiological and biochemical characteristics. Sequences of the 16S rRNA gene obtained by PCR were identical for all isolates. These results indicate that the bacterial isolates from potato scabby tissue belong to S. acidiscabies. Furthermore, the effectiveness of fluazinam, both in vitro and under greenhouse and field conditions, represents a possible

option for chemical control of potato common scab disease. While our results suggest that spraying at seeding is effective in controlling common scab, future studies to combine this treatment with seed dressing before planting will be conducted to determine if there is an increase in disease control.

Resumen

Se investigó la patogenicidad de diez aislamientos bacterianos en papa, rábano, sanahoria y betabel, incluyendo la sensibilidad y la eficacia de control del patógeno. Se identificó a los aislamientos mediante métodos morfológicos, bioquímicos, y moleculares. Todos los aislamientos fueron patogénicos en rábano, zanahoria y betabel, y fueron altamente virulentos en papa. Aunque los aislamientos se obtuvieron de diferentes lugares en el Valle del fuerte (Sinaloa, México) fueron similares en sus características morflógicas, fisiológicas y bioquímicas. Las secuencias del gen 16S rRNA obtenidas por PCR fueron idénticas para todos los aislamientos. Estos resultados indican que los aislamientos bacterianos de tejido de la roña en papa pertenecen a S. acidiscabies. Aún más, la efectividad de fluazinam, tanto in vitro como bajo condiciones de invernadero y campo, representa una opción posible para el control químico de la enfermedad de la roña común en papa. Mientras que nuestros resultados sugieren que la aspersión en la siembra es efectiva en el control de la roña común, se conducirán estudios a futuro para combinar este tratamiento con cubrimiento a la semilla antes de la siembra para determinar si hay un aumento en el control de la enfermedad.

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Authors and Affiliations

  1. Instituto Politécnico Nacional, CIIDIR, Departamento de Biotecnología Agrícola, Unidad Sinaloa, Blvd. Juan de Dios Bátiz Paredes No. 250, San Joachín, C.P, 81101, Guasave, Sinaloa, Mexico

    María Elena Santos-Cervantes, Mariela Guadalupe Espinoza-Mancillas & Norma Elena Leyva-López

  2. Universidad de Occidente, Departamento de ciencias Biológicas, Unidad Los Mochis, C.P, 81223, Los Mochis, Sinaloa, Mexico

    Ruben Felix-Gastelum

  3. Junta Local de Sanidad Vegetal del Valle del Fuerte, Lázaro Cárdenas 315 Pte, Col. Centro, C.P, 81200, Los Mochis, Sinaloa, Mexico

    Gabriel Herrera-Rodríguez

  4. Universidad de Occidente, Departamento de Ciencias Biológicas, Unidad Guasave, Av. Universidad S/N Fracc. Villa Universidad, C.P, 81048, Guasave, Sinaloa, Mexico

    Arlene Guadalupe Mora-Romero

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  1. María Elena Santos-Cervantes
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  2. Ruben Felix-Gastelum
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  3. Gabriel Herrera-Rodríguez
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Correspondence to Ruben Felix-Gastelum.

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Santos-Cervantes, M.E., Felix-Gastelum, R., Herrera-Rodríguez, G. et al. Characterization, Pathogenicity and Chemical Control of Streptomyces acidiscabies Associated to Potato Common Scab. Am. J. Potato Res. 94, 14–25 (2017). https://doi.org/10.1007/s12230-016-9541-5

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