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Anastomosis Group and Pathogenicity of Rhizoctonia solani Associated with Stem Canker and Black Scurf of Potato in Heilongjiang Province of China

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Abstract

From 2012 to 2015, a total of 226 isolates of Rhizoctonia solani were collected from the stem cankers on potato stems and sclerotia on tubers from different potato cultivation areas of Heilongjiang Province, China. These isolates were assigned to the anastomosis group (AG) by performing conventional PCR assays using previously published primers for ITS-rDNA regions, as well as by observing hyphal interactions where appropriate. Most of the isolates were assigned to AG-3PT (58.85 %), and several were assigned to AG-5 (21.68 %), AG-2-1 (7.08 %) and AG-4 (12.39 %). Pathogenicity tests showed that the AG-3 and AG-5 isolates had the highest virulence, and the disease indices were 1.96a and 2.47a for stem and 1.48a and 1.6a for root (P < 0.05) after analyzed by LSD multiple comparisons, respectively. Both two isolates consistently caused large brown lesions with sunken on the potato stems and roots in in vitro and greenhouse experiments. This is the first detailed report on the AG composition, variability and pathogenicity of R. solani isolates associated with stem cankers and black scurf found on potatoes cultivated in Heilongjiang Province.

Resumen

Del 2012 al 2015, se colectó un total de 226 aislamientos de Rhizoctonia solani de las lesiones del tallo y esclerocios de los tubérculos de diferentes áreas de cultivo de papa de la provincia de Heilongjiang, China. Estos aislamientos se asignaron al grupo de anastomosis (AG) mediante la conducción de ensayos convencionales de PCR utilizando iniciadores previamente publicados de las regiones ITS-rDNA, así como mediante la observación de las interacciones de las hifas cuando fue apropiado. La mayoría de los aislamientos fueron asignados a AG-3PT (58.85 %) y varios a AG-5 (21.68 %), AG-2-1 (7.08 %) y AG-4 (12.39 %). Las pruebas de patogenicidad mostraron que los aislamientos AG-3 y AG-5 tenían la virulencia más alta, y los índices de la enfermedad fueron 1.96a y 2.47a para tallo y de1.48a y 1.6a para raíz (P < 0.05) después de ser analizados mediante comparaciones múltiples LSD respectivamente. Ambos tipos de aislamientos causaron consistentemente grandes lesiones cafés con hundimientos en los tallos y raíces de papa en experimentos in vitro y de invernadero. Este es el primer reporte detallado en la composición de AG, variabilidad y patogénesis de aislamientos de R. solani asociados con cáncer del tallo y la costra negra encontrados en papa cultivada en la Provincia de Heilongjiang.

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Acknowledgments

We gratefully acknowledge Mr. Vinh Hong Le (Norwegian Institute of Bioeconomy Research, Oslo, Norway) for his constructive reviews of the manuscript. This work was supported by the Sino-Norway Cooperative Research on Monitoring and Control of Potato Soil-borne pests (2013DFA31970) and the National Science and Technology Support Project (2012BAD06B02).

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Authors and Affiliations

  1. Virus-Free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China

    Shuai Yang, Fanxiang Min, Wenzhong Wang, Qi Wei, Mei Guo, Yunfei Gao, Xuezhi Dong & Dianqiu Lu

  2. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, 150001, China

    Shuai Yang & Dianqiu Lu

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  1. Shuai Yang
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Yang, S., Min, F., Wang, W. et al. Anastomosis Group and Pathogenicity of Rhizoctonia solani Associated with Stem Canker and Black Scurf of Potato in Heilongjiang Province of China. Am. J. Potato Res. 94, 95–104 (2017). https://doi.org/10.1007/s12230-016-9535-3

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