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Anastomosis group and pathogenicity of Rhizoctonia solani associated with stem canker and black scurf of potato in China

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Abstract

Three hundred and fifty six isolates of Rhizoctonia solani were obtained from potato stems and tubers in the main potato growing regions of China between 2011 and 2014. On the basis of the hyphal anastomosis and sequence analysis of the internal transcribed spacers of the ribosomal DNA, 249 isolates (69.94 %) were identified to anastomosis group (AG)-3 PT, 44 (12.36 %) to AG-5, 39 (10.96 %) to AG-4-HGI, 12 (3.37 %) to AG-4-HGII, seven (1.97 %) to AG-4-HGIII, three (0.84 %) to AG-2-1, one (0.28 %) to AG-1-IB, and one (0.28 %) to AG-11. All these AGs could be isolated from potato cankered subterranean stems, whereas isolates obtained from sclerotia on tubers just belonged to AG-3 PT, AG-4-HGI, and AG-5. The selected 56 isolates representing different AGs and subgroups were able to cause brown, dry lesions on potato subterranean stems with disease incidences ranging from 21.43 to 92.87 %. The lesions caused by AG-3 PT isolates were deeply sunken, while those caused by isolates of other AGs were slightly sunken. Taken together, AG-3 PT of R. solani was the predominant pathogen causing stem canker and black scurf on potatoes in China. To our knowledge, this is the first detailed report of the AG composition of R. solani populations causing potato disease in China.

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Acknowledgments

We are grateful to N. Kondo (Graduate School of Agriculture, Hokkaido University, Sapporo, Japan) for kindly providing reference strains. This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1042). Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement.

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  1. Department of Plant Pathology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

    Yonggang Yang, Can Zhao, Zejian Guo & Xuehong Wu

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  1. Yonggang Yang
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Correspondence to Xuehong Wu.

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Yang, Y., Zhao, C., Guo, Z. et al. Anastomosis group and pathogenicity of Rhizoctonia solani associated with stem canker and black scurf of potato in China. Eur J Plant Pathol 143, 99–111 (2015). https://doi.org/10.1007/s10658-015-0668-x

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