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Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions

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Abstract

The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.

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References

  • Amore A, Giacobbe S, Faraco V (2013) Regulation of cellulase and hemicellulase gene expression in fungi. Curr Genomics 14:230–249

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bao W, Lymar E, Renganathan V (1994) Optimization of cellobiose dehydrogenase and β-glucosidase production by cellulose-degrading cultures of Phanerochaete chrysosporium. Appl Microbiol Biotechnol 42:642–646

    Article  CAS  Google Scholar 

  • Chen X, Luo Y, Yu H, Sun Y, Wu H, Song S, Hu S, Dong Z (2014) Transcriptional profiling of biomass degradation-related genes during Trichoderma reesei growth on different carbon sources. J Biotechnol 173:59–64

    Article  CAS  PubMed  Google Scholar 

  • Deshpande V, Eriksson KE, Pettersson B (1978) Production, purification and partial characterization of 1, 4-β-glucosidase enzymes from Sporotrichum pulverulentum. Eur J Biochem 90:191–198

    Article  CAS  PubMed  Google Scholar 

  • Dons JJM, De Vries OMH, Wessels JGH (1979) Characterization of the genome of the basidiomycete Schizophyllum commune. Biochim Biophys Acta 563:100–112

    Article  CAS  PubMed  Google Scholar 

  • Gao L, Gao F, Jiang X, Zhang C, Zhang D, Wang L, Wu G, Chen S (2014) Biochemical characterization of a new β-glucosidase (Cel3E) from Penicillium piceum and its application in boosting lignocelluloses bioconversion and forming disaccharide inducers: new insights into the role of β-glucosidase. Process Biochem 49:768–774

    Article  CAS  Google Scholar 

  • Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781–788

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hong J, Tamaki H, Kumagai H (2007) Cloning and functional expression of thermostable β-glucosidase gene from Thermoascus aurantiacus. Appl Microbiol Biotechnol 73:1331–1339

    Article  CAS  PubMed  Google Scholar 

  • Jørgensen H, Mørkeberg A, Krogh KBR, Olsson L (2004) Growth and enzyme production by three Penicillium species on monosaccharides. J Biotechnol 109:295–299

    Article  PubMed  Google Scholar 

  • Krogh KB, Harris PV, Olsen CL, Johansen KS, Hojer-Pedersen J, Borjesson J, Olsson L (2010) Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum. Appl Microbiol Biotechnol 86:143–154

    Article  CAS  PubMed  Google Scholar 

  • Lee YM, Lee H, Kim JS, Lee J, Ahn BJ, Kim G-H, Kim J-J (2014) Optimization of medium components for β-glucosidase production of Schizophyllum commune KUC9397 and enzymatic hydrolysis of lignocellulosic biomass. Bioresources 9:4358–4368

    Google Scholar 

  • Li B, Renganathan V (1998) Gene cloning and characterization of a novel cellulose-binding β-glucosidase from Phanerochaete chrysosporium. Appl Environ Microbiol 64:2748–2754

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lo AC, Barbier JR, Willick GE (1990) Kinetics and specificities of two closely related β-glucosidases secreted by Schizophyllum commune. Eur J Biochem 192:175–181

    Article  CAS  PubMed  Google Scholar 

  • Lo AC, Willick G, Bernier R, Desrochers M (1988) Purification and assay of beta-glucosidase from Schizophyllum commune. Methods Enzymol 160:432–437

    Article  CAS  Google Scholar 

  • Margolles-Clark E, Ilmén M, Penttilä M (1997) Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources. J Biotechnol 57:167–179

    Article  CAS  Google Scholar 

  • Ohm RA, De Jong JF, Lugones LG, Aerts A, Kothe E, Stajich JE, de Vries RP et al (2010) Genome sequence of the model mushroom Schizophyllum commune. Nat Biotechnol 28:957–963

    Article  CAS  PubMed  Google Scholar 

  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Smith MH, Gold MH (1979) Phanerochaete chrysosporium β-glucosidases: induction, cellular localization, and physical characterization. Appl Environ Microbiol 37:938–942

    CAS  PubMed  PubMed Central  Google Scholar 

  • Steenbakkers PJ, Harhangi HR, Bosscher MW, van der HOOFT MM, Keltjens JT, van der DRIFT C, Vogels GD (2003) β-glucosidase in cellulosome of the anaerobic fungus Piromyces sp. strain E2 is a family 3 glycoside hydrolase. Biochem J 370:963–970

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Suto M, Tomita F (2001) Induction and catabolite repression mechanisms of cellulase in fungi. J Biosci Bioeng 92:305–311

    Article  CAS  PubMed  Google Scholar 

  • Sweeney MD, Xu F (2012) Biomass converting enzymes as industrial biocatalysts for fuels and chemicals: recent developments. Catalysts 2:244–263

    Article  CAS  Google Scholar 

  • Whitaker DR (1951) Studies in the biochemistry of cellulolytic microorganisms: I. Carbon balances of wood-rotting fungi in surface culture. Can J Bot 29:159–175

    Article  CAS  Google Scholar 

  • Willick GE, Morosoli R, Seligy VL, Yaguchi M, Desrochers M (1984) Extracellular proteins secreted by the basidiomycete Schizophyllum commune in response to carbon source. J Bacteriol 159:294–299

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wong DW, Chan VJ, McCormack AA, Hirsch J, Biely P (2012) Functional cloning and expression of the Schizophyllum commune glucuronoyl esterase gene and characterization of the recombinant enzyme. Biotechnol Res Int 2012:1–7

    Article  Google Scholar 

  • Xu R, Teng F, Zhang C, Li D (2011) Cloning of a gene encoding β-glucosidase from Chaetomium thermophilum CT2 and its expression in Pichia pastoris. J Mol Microbiol Biotechnol 20:16–23

    Article  PubMed  Google Scholar 

  • Yoshida M, Igarashi K, Kawai R, Aida K, Samejima M (2004) Differential transcription of β-glucosidase and cellobiose dehydrogenase genes in cellulose degradation by the basidiomycete Phanerochaete chrysosporium. FEMS Microbiol Lett 235:177–182

    CAS  PubMed  Google Scholar 

  • Zhu N, Liu J, Yang J, Lin Y, Yang Y, Ji L, Li M, Yuan H (2016) Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system. Biotechnol Biofuels 9:1

    Article  Google Scholar 

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Acknowledgements

This study was supported by a Korea University Grant.

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Authors and Affiliations

  1. Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea

    Young Min Lee, Hanbyul Lee, Young Mok Heo, Hwanhwi Lee, Joo-Hyun Hong & Jae-Jin Kim

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  1. Young Min Lee
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  2. Hanbyul Lee
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  3. Young Mok Heo
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  4. Hwanhwi Lee
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  5. Joo-Hyun Hong
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  6. Jae-Jin Kim
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Correspondence to Jae-Jin Kim.

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Lee, Y.M., Lee, H., Heo, Y.M. et al. Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions. Folia Microbiol 62, 191–196 (2017). https://doi.org/10.1007/s12223-016-0484-5

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  • DOI: https://doi.org/10.1007/s12223-016-0484-5

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