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Electrospun Composite Nanofibers Based on PLA/Artesunate-loaded Citrate-functionalized Hydroxyapatite for Boosting in vitro Anticancer Efficacy and Drug Delivery of Artesunate

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Abstract

The development of a facile method for preparing electrospun artesunate-loaded inorganic/organic composite nanofiber drug delivery systems will significantly enhance the therapeutic applications of artesunate as a novel anticancer medication. Herein, artesunate- loaded citrate-functionalized hydroxyapatite nanorods (ART@Cit-HAp NRs) were prepared, and then encapsulated into PLA nanofibers (ART@Cit-HAp NRs/PLA composite nanofibers) via the electrospinning method. The produced Cit-HAp NRs, ART@Cit-HAp NRs and ART@Cit-HAp NRs/PLA composite nanofibers were characterized using a variety of comprehensive techniques. Electrospun PLA nanofibers have a smooth surface and a homogeneous diameter without any grains on the surface. The as-prepared citrate functionalized hydroxyapatite nanorods showed the high drug-loading efficiency of ∼92 %. The mechanism of pseudo-second-order adsorption is the most prominent model for ART adsorption on Cit-HAp NRs. The in vitro ART release from the composite nanofibers (ART@Cit-HAp NRs/PLA composite nanofibers) was investigated and compared to ART@Cit-HAp NRs under different pH conditions. The ART released from the ART@Cit-HAp NRs/PLA composite nanofibers in a sustained, slow rate was in contrast to Cit-HAp NRs and dependent on the pH of the medium. The in vitro antitumor activity of nanoformulations were evaluated on two colon cancer (CaCo-2 and HCT 116) cell lines. The findings revealed that ART@Cit-HAp NRs/PLA composite nanofibers demonstrated higher anti-proliferating activity compared to free ART, and the pH-triggered release of ART@Cit-HAp NRs/PLA composite nanofibers are interesting prospects for colon cancer treatment.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt

    Nehal Salahuddin

  2. Department of Chemistry, Faculty of Science, Nanotechnology Center, Kafr Elsheikh University, Kafr Elsheikh, 33516, Egypt

    Ebtisam Mohamed Ibrahim

  3. Institute of Nanoscience & Nanotechnology, Kafr Elsheikh University, Kafr Elsheikh, 33516, Egypt

    Maged El-Kemary

Authors
  1. Nehal Salahuddin
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  2. Ebtisam Mohamed Ibrahim
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  3. Maged El-Kemary
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Correspondence to Nehal Salahuddin or Maged El-Kemary.

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Salahuddin, N., Ibrahim, E.M. & El-Kemary, M. Electrospun Composite Nanofibers Based on PLA/Artesunate-loaded Citrate-functionalized Hydroxyapatite for Boosting in vitro Anticancer Efficacy and Drug Delivery of Artesunate. Fibers Polym 23, 3415–3426 (2022). https://doi.org/10.1007/s12221-022-4017-3

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  • DOI: https://doi.org/10.1007/s12221-022-4017-3

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