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Sevoflurane Suppresses the Proliferation, Migration and Invasion of Colorectal Cancer Through Regulating Circ_0000423/miR-525-5p/SGPP1 Network

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Abstract

Introduction

Sevoflurane (SEV) has been shown to inhibit the malignant progression in many cancers, including colorectal cancer (CRC). However, it is not clear whether SEV regulates the progression of CRC by mediating the circular RNA (circRNA) axis.

Methods

Different concentrations of SEV were used to treat CRC cells. Cell proliferation, migration and invasion were determined by cell counting kit 8 assay, colony formation assay and transwell assay. The expression of circ_0000423, microRNA (miR)-525-5p and sphingosine-1-phosphate phosphatase 1 (SGPP1) mRNA was measured by quantitative real-time PCR. Cell apoptosis was assessed using flow cytometry, and protein expression was measured by western blot analysis. Dual-luciferase reporter assay and RIP assay were performed to confirm the interactions among circ_0000423, miR-525-5p and SGPP1. Animal experiments were performed to explore the effect of SEV and circ_0000423 on CRC tumorigenesis.

Results

SEV could inhibit CRC cell proliferation, migration and invasion. Circ_0000423 was upregulated in CRC and its expression could be reduced by SEV. Overexpressed circ_0000423 reversed the inhibitory effect of SEV on CRC cell proliferation, migration and invasion and the promotion effect on cell apoptosis. MiR-525-5p could be sponged by circ_0000423, and its overexpression also abolished the regulation of circ_0000423 on the progression of SEV-treated CRC cells. In addition, SGPP1 was confirmed to be a target of miR-525-5p, and its expression was positively regulated by circ_0000423. MiR-525-5p inhibitor promoted CRC cell progression under the treatment of SEV, while these effects could be overturned by SGPP1 silencing. Furthermore, the inhibition effect of SEV on CRC tumorigenesis also could be abolished by overexpressing circ_0000423.

Conclusion

Our results showed that SEV inhibited CRC progression through the regulation of circ_0000423/miR-525-5p/SGPP1 axis.

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Funding

There is no funding.

Conflict of interest

Xiaofang Kang, Xiaocong Li, Yanli Li declare that they have no conflicts of interest.

Ethical Approval

All tissues samples were collected with written informed consent in accordance with the Declaration of Helsinki and with the approval of the Ethics Committee of The 980 Hospital of the joint logistics support force of the Chinese people's Liberation Army.

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Authors and Affiliations

  1. Department of Anesthesiology, The 980 Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army, No. 398, Zhongshan West Road, Shijiazhuang City, 050000, Hebei Province, China

    Xiaofang Kang, Xiaocong Li & Yanli Li

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  1. Xiaofang Kang
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  2. Xiaocong Li
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  3. Yanli Li
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Correspondence to Xiaofang Kang.

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Associate Editor Michael R. King oversaw the review of this article.

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Supplementary Information

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12195_2021_717_MOESM1_ESM.tif

Supplementary Fig. 1 The partial color figures. The representative picture for Fig.2H (A), Fig. 2J (B) and Fig. 2K (C) (TIF 6901 kb)

12195_2021_717_MOESM2_ESM.tif

All protein quantification results. The protein quantificationresults for Fig. 2L-M (A-B), Fig. 4G-H (C-D), Fig. 5G (E), Fig. 5H (F), Fig. 5K (G),Fig. 5L (H), Fig. 5N (I), Fig. 6G-H (J-K), and Fig. 7E (L). *p < 0.05 (TIF 1223 kb)

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Kang, X., Li, X. & Li, Y. Sevoflurane Suppresses the Proliferation, Migration and Invasion of Colorectal Cancer Through Regulating Circ_0000423/miR-525-5p/SGPP1 Network. Cel. Mol. Bioeng. 15, 219–230 (2022). https://doi.org/10.1007/s12195-021-00717-5

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