Abstract
Adriamycin (ADR) is an important chemotherapeutic drug, but it has serious side effects such as hepatotoxicity. This study aimed to evaluate whether N-acetylcysteine (NAC) has hepatoprotective effects against ADR-induced hepatotoxicity in rats. In addition, it was aimed to determine how Meteorin-Like (MtrnL), which has pleiotropic effects on immunology, inflammation, and metabolism, is affected by ADR and/or NAC applications in liver tissue. 28 rats were randomly assigned to one of four equal groups in the study: control (no treatment), NAC (150 mg/kg/day of NAC intraperitoneally (i.p), ADR (15 mg/kg only on the first day of the experiment), and ADR + NAC (ADR 15 mg/kg on the first day of the experiment + 150 mg/kg/day NAC i.p). After 15 days, liver enzyme levels in serum, oxidant/antioxidant parameters in liver tissue, histopathological changes, caspase 3 (Casp3) and heat shock protein 70 (HSP-70) immunoreactivities, and MtrnL levels were examined. Histopathological changes, liver enzyme levels, as well as HSP-70, and Casp3 immunoreactivities increased due to ADR application. Additionally, MtrnL levels in liver tissue were significantly increased as a result of ADR application. However, it was detected that the NAC application significantly regulated the ADR-induced changes. Furthermore, it was determined that NAC administration regulated the changes in ADR-induced oxidative stress parameters. We propose that NAC may exert a hepatoprotective effect by regulating ADR-induced altered oxidative stress parameters, MtrnL levels, Casp3, and HSP-70 immunoreactivities in the liver.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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This study was supported by Fırat University Scientific Research Projects Unit, Elazığ, TURKEY with project number TF.21.11.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Sercan Kaya, Tuba Yalcın, Ahmet Tektemur and Tuncay Kuloglu. The first draft of the manuscript was written by Sercan Kaya and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This study has been approved by the decision of Fırat University Animal Experiments Ethics Committee with the number 2831 and dated 05.07.2021.
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Kaya, S., Yalcın, T., Tektemur, A. et al. N-Acetylcysteine may exert hepatoprotective effect by regulating Meteorin-Like levels in Adriamycin-induced liver injury. Cell Stress and Chaperones 28, 849–859 (2023). https://doi.org/10.1007/s12192-023-01376-3
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DOI: https://doi.org/10.1007/s12192-023-01376-3