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Possible protective activity of n-acetyl cysteine against cisplatin‑induced hepatotoxicity in rats

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Abstract

CP is one of the most widely used antineoplastic agents. However, its clinical application is very limited due to its severe toxic effects. The present study aimed to reveal the effects of NAC, which exhibits broad biological activities in reducing CP-induced liver damage, in consideration of biochemical, genetic, and histopathological findings. Twenty-eight wistar rats were randomly divided into four groups of seven animals. A dose of saline was administered (i.p.) to the control group for 5 days. One dose of NAC (200 mg/kg) was administered to the NAC group for 5 days (i.p.). To the NAC + CP group, a dose of CP (7.5 mg/kg) was administered on days 2 and 5 of the experiment, a dose of NAC (200 mg/ kg) (i.p.) was administered for 5 day of the experiment. CP (7.5 mg/kg) was administered to the CP group on days 2 and 5 of the experiment. At the end of the experiment, the biochemical, histological, and mRNA expression analyses of the liver tissues isolated from all the rats were performed. A statistically significant decrease was observed in the AST and ALT enzyme activities in Group NAC + CP compared to Control and CP groups. In addition, it was determined that the NAC administration reduced CP-induced inflammation by increasing the level of NF-κB and decreased CP-caused oxidative stress by decreasing the GPx level. Moreover, the histopathological analyses showed that NAC improved liver morphology. It was revealed by Western blotting analysis that NAC promoted Bcl-2 signaling and decreased p53 signaling. The findings herein showed that NAC could help alleviate hepatotoxicity, a serious therapeutic complication, by reducing CP-induced oxidative stress and playing an effective part in the regulation of apoptotic markers.

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Acknowledgements

This study was financially supported by the Office of Scientific Research Projects, Çanakkale Onsekiz Mart University (Project Number: TSA-2019-2856).

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Authors and Affiliations

  1. Faculty of Medicine, Department of Biophysics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Özlem Coşkun & Özlem Öztopuz

  2. Faculty of Medicine, Department of Histology-Embryology, İzmir Democracy University, İzmir, Turkey

    Başak Büyük

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  1. Özlem Coşkun
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  2. Özlem Öztopuz
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  3. Başak Büyük
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Correspondence to Özlem Coşkun.

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This study was approved by the animal ethics committee of Çanakkale Onsekiz Mart University in accordance with ethical guidelines for the Care and Use of Laboratory Animals. (Decision No: 2020/01-01).

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Coşkun, Ö., Öztopuz, Ö. & Büyük, B. Possible protective activity of n-acetyl cysteine against cisplatin‑induced hepatotoxicity in rats. Mol Biol Rep 48, 637–644 (2021). https://doi.org/10.1007/s11033-020-06111-0

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  • DOI: https://doi.org/10.1007/s11033-020-06111-0

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