Abstract
pH has a great impact on the distribution, growth, behavior, and physiology in many aquatic animals. Here, we analyzed miRNA expression profiles of Chinese shrimp (Fenneropenaeus chinensis) from control pH (8.2) and low pH (6.5)-treated shrimp. Expression analysis identified 6 known miRNAs and 23 novel miRNAs with significantly different expression between control pH 8.2 and low pH 6.5; the predicted target genes of differentially expressed miRNAs were significantly enriched in organic acid metabolic process, oxidoreductase activity, coenzyme binding, cofactor binding, and collagen trimer. Moreover, target genes were significantly enriched in several Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including citrate cycle, pyruvate metabolism, cytokine-cytokine receptor interaction, tight junction, carbon metabolism, etc. Our survey expanded the number of known shrimp miRNAs and provided comprehensive information about miRNA response to low pH stress.
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Funding
This work was supported by grants from the Program of Taishan Industrial Experts (Grant No. LNJY2015002), the National Natural Science Foundation of China (Grant No. 31772842), and the China Agriculture Research System (Grant No. CARS-48).
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He, Y., Li, Z., Zhang, H. et al. Genome-wide identification of Chinese shrimp (Fenneropenaeus chinensis) microRNA responsive to low pH stress by deep sequencing. Cell Stress and Chaperones 24, 689–695 (2019). https://doi.org/10.1007/s12192-019-00989-x
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DOI: https://doi.org/10.1007/s12192-019-00989-x