Abstract
To understand the potential association of heat stress resistance with HspB1 induction by aspirin (ASA) in chicken myocardial cells, variations of HspB1 expression and heat stressed-induced damage of myocardial cells after ASA administration were studied in primary cultured myocardial cells. Cytopathological lesions as well as damage-related enzymes, such as creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH), indicated the considerable protective ability of ASA pre-treatment against acute heat stress. Immunostaining assays showed that heat stress caused HspB1 to relocate into the nucleus, while ASA did not. ELISA analysis, revealed that HspB1 expression induced by ASA averaged 45.62-fold higher than that of the control. These results indicated that the acute heat-stressed injuries were accompanied by comparatively lower HspB1 expression caused by heat stress in vitro. ASA pre-treatment induced a level of HspB1 presumed to be sufficient to protect myocardial cells from acute heat stress in the extracorporal model, although more detailed mechanisms will require further investigation.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (31372403), the Natural Science Foundation of the Jiangsu Province (Grant No. BK20140107), the Postgraduate Student Research and Innovation Project of Jiangsu Province (KYLX15_0558), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Sino-German Agricultural Cooperation Project of the Federal Ministry of Food, the Agriculture and Consumer Production, Berlin, Germany.
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Di Wu and Miao Zhang contributed equally to this work.
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Wu, D., Zhang, M., Xu, J. et al. In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells. Cell Stress and Chaperones 21, 405–413 (2016). https://doi.org/10.1007/s12192-016-0666-8
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DOI: https://doi.org/10.1007/s12192-016-0666-8