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Acrylamide in Cookie Samples: Analysis Using an Efficient Co-Derivatization Coupled with Sensitive Microextraction Method Followed by Gas Chromatography-Mass Spectrometry

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Abstract

Acrylamide, a “possible carcinogen” compound, has been subjected to analytical method development and evaluation in carbohydrate-rich cookie samples in this research. Cookies with different formula and consequently different acrylamide content were evaluated using microextraction method coupled with gas chromatography-mass spectrometry. Xanthydrol was selected as an efficient derivatization reagent. Important parameters in the extraction step such as the volume of extraction solvent, volume of dispersive solvent, amount of salt, and sample pH were optimized using central composite design. The calibration curve showed good linearity (R2 > 0.998) for acrylamide in the range of 2–500 ng g−1. The limits of detection and quantitation were 0.6 ng g−1 and 1.9 ng g−1, respectively. The relative standard deviation for the six analyses was 9.2%, and relative recoveries of acrylamide in spiked cookie samples were 89–95%. The proposed method demonstrated high recovery, sensitivity, and good enrichment factor for acrylamide determination of cookies with low and high acrylamide content.

Determining acrylamide in cookie samples using HDS-DLLME-GC-MS

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Acknowledgement

This study has been supported by the Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. We gratefully appreciate their assistance.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Motahareh Elahi, Marzieh Kamankesh, Abdorreza Mohammadi & Sahar Jazaeri

  2. Research Laboratory of Spectroscopy & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, Iran

    Marzieh Kamankesh

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  1. Motahareh Elahi
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  2. Marzieh Kamankesh
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  3. Abdorreza Mohammadi
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  4. Sahar Jazaeri
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Correspondence to Abdorreza Mohammadi or Sahar Jazaeri.

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Motahareh Elahi declares that she has no conflict of interest. Marzieh Kamankesh declares that she has no conflict of interest. Abdorreza Mohammadi declares that he has no conflict of interest. Sahar Jazaeri declares that she has no conflict of interest.

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Elahi, M., Kamankesh, M., Mohammadi, A. et al. Acrylamide in Cookie Samples: Analysis Using an Efficient Co-Derivatization Coupled with Sensitive Microextraction Method Followed by Gas Chromatography-Mass Spectrometry. Food Anal. Methods 12, 1439–1447 (2019). https://doi.org/10.1007/s12161-019-01479-7

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