Skip to main content
SpringerLink
Log in

Direct Competitive Biomimetic Immunoassay Based on Quantum Dot Label for Simultaneous Determination of Two Pesticide Residues in Fruit and Vegetable Samples

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

In this study, trichlorfon and chlorpyrifos conjugated to CdSe/ZnS quantum dots with different emission wavelengths were adopted as labels. A hydrophilic imprinted film synthesized directly on the surface of a 96-well plate was used as the antibody. A direct competitive biomimetic immunoassay method for simultaneous determination of trichlorfon and chlorpyrifos in fruits and vegetables was developed. Under optimal conditions, the limit of detection of this method was 18.0 μg/L for chlorpyrifos (55 mg/kg for sample) and 19.0 μg/L for trichlorfon (45 mg/kg for sample). Orange and carrot samples spiked with chlorpyrifos and trichlorfon were extracted and determined by this method with recoveries ranging from 77.8 to 92.0%. The method was applied for detection of chlorpyrifos and trichlorfon residues in banana and apple samples, and the results correlated well with those obtained using gas chromatography.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Aulakh JS, Malik AK, Varinder K, Philippe SK (2005) A review on solid phase micro extraction-high performance liquid chromatography (SPME-HPLC) analysis of pesticides. Crit Rev Anal Chem 35(1):71–85

    Article  CAS  Google Scholar 

  • Bakas I, Oujji NB, Moczko E, Istamboulie G, Piletsky S, Piletska E, Ait-Ichou I, Ait-Addi E, Noguer T, Rouillon R (2012) Molecular imprinting solid phase extraction for selective detection of methidathion in olive oil. Anal Chim Acta 734(13):99–105

    Article  CAS  Google Scholar 

  • Cao Z, Li H, Lau C, Zhang Y (2011) Cross-talk-free simultaneous fluoroimmunoassay of two biomarkers based on dual-color quantum dots. Anal Chim Acta 698(1):44–50

    Article  CAS  Google Scholar 

  • Chen H, Chen RW, Feng R, Li SQ (2009) Simultaneous analysis of carbamate and organophosphorus pesticides in water by single-drop microextraction coupled with GC-MS. Chromatogra 70(1–2):165–172

    Article  CAS  Google Scholar 

  • Chianella I, Guerreiro A, Moczko E, Caygill JS, Piletska EV, Whitcombe MJ (2013) Direct replacement of antibodies with molecularly imprinted polymer nanoparticles in ELISA-development of a novel assay for vancomycin. Anal Chem 85(17):8462–8468

    Article  CAS  Google Scholar 

  • Esteve-Turrillas FA, Abad-Fuentes A (2013) Applications of quantum dots as probes in immunosensing of small-sized analytes. Biosens Bioelectron 41(1):12–29

    Article  CAS  Google Scholar 

  • Harshit D, Charmy K, Nrupesh P (2017) Organophosphorus pesticides determination by novel HPLC and spectrophotometric method. Food Chem 230:448–453

    Article  CAS  Google Scholar 

  • Hooshyar Z, Bardajee GR (2017) Fluorescence enhancement of glutathione capped CdTe/ZnS quantum dots by embedding into cationic starch for sensitive detection of rifampicin. Spectrochim Acta A 173:144–150

    Article  CAS  Google Scholar 

  • Khalilizanjani MR, Yamini Y, Yazdanfar N, Shariati S (2008) Extraction and determination of organophosphorus pesticides in water samples by a new liquid phase microextraction-gas chromatography-flame photometric detection. Anal Chim Acta 606(2):202–208

    Article  CAS  Google Scholar 

  • Liang X, Xie R, Wang CM, Gui WJ, Zhu GN (2013) Development of a broad-selective immunoassay for multi-residue determination of type II. Pyrethroids in west lake water. Food Agr Immunol 24(1):59–78

    Article  CAS  Google Scholar 

  • Mahpishanian S, Sereshti H (2016) Three-dimensional graphene aerogel-supported iron oxide nanoparticles as an efficient adsorbent for magnetic solid phase extraction of organophosphorus pesticide residues in fruit juices followed by gas chromatographic determination. J Chromatogr A 1443:43–53

    Article  CAS  Google Scholar 

  • Manclus J, Primo J, Montoya A (1994) Development of a chlorpyrifos immunoassay using antibodies obtained from a simple hapten design. Journal of Agricultural & Food Chemistr 42(6):1257–1260

    Article  CAS  Google Scholar 

  • Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4(6):435–446

    Article  CAS  Google Scholar 

  • Meng L, Qiao XG, Xu ZX, Xin JH, Wang L (2012) Development of a direct competitive biomimetic enzyme-linked immunosorbent assay based on a hydrophilic molecularly imprinted membrane for the determination of trichlorfon residues in vegetables. Food Anal Method 5(5):1229–1236

    Article  Google Scholar 

  • Meng XW, Wei JF, Ren XL, Ren J, Tang FQ (2013) A simple and sensitive fluorescence biosensor for detection of organophosphorus pesticides using H2O2-sensitive quantum dots/bi-enzyme. Biosens Bioelectron 47(18):402–407

    Article  CAS  Google Scholar 

  • Pundir CS, Chauhan N (2012) Acetylcholinesterase inhibition-based biosensors for pesticide determination: a review. Anal Biochem 429(1):19–31

    Article  CAS  Google Scholar 

  • Qian L, Li HT, Zhang YY, Yao SZ (2015) Upconversion nanoparticle-based fluorescence resonance energy transfer assay for organophosphorus pesticides. Biosens Bioelectron 68:168–174

    Article  Google Scholar 

  • Salvador IM, Frenich AG, González FJE, Vidal JLM (2006) Determination of organophosphorus pesticides in vegetables by GC with pulsed flame-photometric detection, and confirmation by MS. Chromatographia 64(11–12):667–672

    Article  Google Scholar 

  • Shi C, Liu XY, Song LY, Qiao XG, Xu ZX (2015) Biomimetic enzyme-linked immunosorbent assay using a hydrophilic molecularly imprinted membrane for recognition and fast determination of trichlorfon and acephate residues in vegetables. Food Anal Method 8(10):2496–2503

    Article  Google Scholar 

  • Song E, Yu M, Wang Y, Hu W, Dan C, Swihart MT (2015) Multi-color quantum dot-based fluorescence immunoassay array for simultaneous visual detection of multiple antibiotic residues in milk. Biosens Bioelectron 72:320–325

    Article  CAS  Google Scholar 

  • Tang YW, Fang GZ, Wang S, Sun JW, Qian K (2013) Rapid determination of metolcarb residues in foods using a biomimetic enzyme-linked immunosorbent assay employing a novel molecularly imprinted polymer film as artificial antibody. J AOAC Int 96(2):453–458

    Article  CAS  Google Scholar 

  • Tang YW, Gao JW, Liu XY, Gao X, Ma T, Lu XN (2017) Ultrasensitive detection of clenbuterol by a covalent imprinted polymer as a biomimetic antibody. Food Chem 228:62–69

    Article  CAS  Google Scholar 

  • Tian XK, Peng H, Li Y, Yang C, Zhou ZX, Wang YX (2017) Highly sensitive and selective paper sensor based on carbon quantum dots for visual detection of TNT residues in groundwater. Sens Actuators B Chem 243:1002–1009

    Article  CAS  Google Scholar 

  • Ueyama J, Saito I, Takaishi A, Nomura H, Mai I, Osaka A (2014) A revised method for determination of dialkylphosphate levels in human urine by solid-phase extraction and liquid chromatography with tandem mass spectrometry: application to human urine samples from Japanese children. Environ Health Prev Med 19(6):405–413

    Article  CAS  Google Scholar 

  • Vonderheide AP, Meija J, Montes-Bayón M, Caruso JA (2003) Use of optional gas and collision cell for enhanced sensitivity of the organophosphorus pesticides by GC-ICP-MS. J Anal Atom Spectrom 18(9):1097–1102

    Article  CAS  Google Scholar 

  • Wang CY, Hou F, Ma YC (2015) Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip. Biosens Bioelectron 68:156–162

    Article  CAS  Google Scholar 

  • Wang JP, Tang WW, Fang GZ, Pan MF, Wang S (2011) Development of a biomimetic enzyme-linked immunosorbent assay method for the determination of methimazole in urine sample. J Chin Chem Soc 58(4):463–469

    Article  CAS  Google Scholar 

  • Wang XL, Tang QH, Wang QQ, Qiao XG, Xu ZX (2014) Study of a molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography for simultaneous determination of trace trichlorfon and monocrotophos residues in vegetables. J Sci Food Agric 94(7):1409–1415

    Article  CAS  Google Scholar 

  • Wei CP, Zhou HQ, Zhou J (2011) Ultrasensitively sensing acephate using molecular imprinting techniques on a surface plasmon resonance sensor. Talanta 83(5):1422–1427

    Article  CAS  Google Scholar 

  • Whitcombe MJ, Kirsch N, Nicholls IA (2014) Molecular imprinting science and technology: a survey of the literature for the years 2004-2011. J Mol Recognit 27(6):297–401

    Article  CAS  Google Scholar 

  • Xia S, Wang XY (2010) Acetylcholinesterase biosensor based on prussian blue-modified electrode for detecting organophosphorous pesticides. Biosens Bioelectron 25(12):2611–2614

    Article  Google Scholar 

  • Xu ZX, Fang GZ, Wang S (2010) Molecularly imprinted solid phase extraction coupled to high-performance liquid chromatography for determination of trace dichlorvos residues in vegetables. Food Chem 119(2):845–850

    Article  CAS  Google Scholar 

  • Zhang C, Cui H, Han Y, Yu F, Shi X (2017) Development of a biomimetic enzyme-linked immunosorbent assay based on molecularly imprinted polymers on paper for the detection of carbaryl. Food Chem 240:893–897

    Article  Google Scholar 

  • Zheng QQ, Chen Y, Fan K, Wu J, Ying YB (2017) Exploring pralidoxime chloride as a universal electrochemical probe for organophosphorus pesticides detection. Anal Chim Acta 982:78–83

    Article  CAS  Google Scholar 

Download references

Funding

This work is financially supported by the National Natural Science Foundation of China (Project No. 31471649).

Dr. Zhixiang Xu has received research grants from the National Natural Science Foundation of China.

Author information

Authors and Affiliations

  1. Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    Qiurui Liu, Jixin Tian, Mingdi Jiang, Xuguang Qiao & Zhixiang Xu

Authors
  1. Qiurui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jixin Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mingdi Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xuguang Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhixiang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhixiang Xu.

Ethics declarations

Conflict of Interest

Qiurui Liu, Jixin Tian, Mingdi Jiang, Xuguang Qiao, and Zhixiang Xu declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, Q., Tian, J., Jiang, M. et al. Direct Competitive Biomimetic Immunoassay Based on Quantum Dot Label for Simultaneous Determination of Two Pesticide Residues in Fruit and Vegetable Samples. Food Anal. Methods 11, 3015–3022 (2018). https://doi.org/10.1007/s12161-018-1285-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-018-1285-z

Keywords

Search

Navigation