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Biomimetic Enzyme-linked Immunosorbent Assay Using a Hydrophilic Molecularly Imprinted Membrane for Recognition and Fast Determination of Trichlorfon and Acephate Residues in Vegetables

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Abstract

This study describes the development of a new direct competitive enzyme-linked immunosorbent assay method with a hydrophilic imprinted film as biomimetic antibody for determining multi-pesticide residues. The imprinted film, which can selectively recognize trichlorfon and acephate, was synthesized directly on the surface of a 96-well plate using 4-(dimethoxyphosphorothioylamino) butanoic acid as the template molecule. This film exhibited antibody-like recognition ability, fast adsorption–desorption dynamics and good stability. Under optimal conditions, the sensitivity (IC50) of the biomimetic enzyme-linked immunosorbent assay (BELISA) method was 12.0 mg/L for trichlorfon and 30.0 mg/L for acephate. The limit of detection (LOD, IC15) was 8.0 μg/L for trichlorfon and 12.0 μg/L for acephate. The developed method was applied to the determination of the trichlorfon and acephate in the spiked asparagus and cucumber samples with recoveries ranging from 72.1 to 92.0 % for trichlorfon and 70.0 to 85.0 % for acephate.

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Acknowledgments

The authors are grateful for financial supports from the National Natural Science Foundation of China (Project No. 31471649) and the Agricultural Science and Technology Achievements Transformation Project of China (Project No. 2013GB2C600276). We have no financial relationship with the organization that sponsored the research. Dr. Zhixiang Xu has received research grants from the National Natural Science Foundation of China and the Agricultural Science and Technology Achievements Transformation Project of China.

Conflict of Interest

Chen Shi, Xueyan Liu, Lingyu Song, Xuguang Qiao, and Zhixiang Xu declare that they have no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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    Authors and Affiliations

    1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

      Chen Shi, Xueyan Liu, Lingyu Song, Xuguang Qiao & Zhixiang Xu

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    1. Chen Shi
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    2. Xueyan Liu
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    3. Lingyu Song
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    4. Xuguang Qiao
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    5. Zhixiang Xu
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    Correspondence to Zhixiang Xu.

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    Xueyan Liu and Chen Shi contributed equally to this work.

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    Shi, C., Liu, X., Song, L. et al. Biomimetic Enzyme-linked Immunosorbent Assay Using a Hydrophilic Molecularly Imprinted Membrane for Recognition and Fast Determination of Trichlorfon and Acephate Residues in Vegetables. Food Anal. Methods 8, 2496–2503 (2015). https://doi.org/10.1007/s12161-015-0141-7

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    • DOI: https://doi.org/10.1007/s12161-015-0141-7

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