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Application of Ultrasound-Assisted Extraction Followed by Solid-Phase Extraction Followed by Dispersive Liquid-Liquid Microextraction for the Determination of Chloramphenicol in Chicken Meat

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Abstract

In the present study, a simple and efficient preconcentration method was developed using ultrasound-assisted extraction followed by solid-phase extraction followed by dispersive liquid-liquid microextraction for the extraction and determination of trace amount of chloramphenicol in chicken meat. The sample was extracted with acetonitrile and EDTA-McIlvaine buffer under ultrasonication, followed by solid-phase extraction and dispersive liquid-liquid microextraction. In order to obtain high extraction efficiency, the parameters affecting the proposed method were evaluated and optimized. Under the optimized conditions, the calibration curve was linear in the range of 0.3–200 μg kg−1 with good linearity (r 2 > 0.995). Finally, applicability of the proposed method was successfully confirmed by extraction and determination of the chloramphenicol in chicken meat samples. Comparing to the traditional methods, the proposed method exhibits high sensitivity and high preconcentration as well as good precision.

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Acknowledgements

Financial support by the Iran National Science Foundation (INSF) (Tehran, Iran) for the support during the period of this research is gratefully acknowledged.

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Authors and Affiliations

  1. Materials and Nuclear Fuel Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran

    Mohammad Rezaee

  2. Department of Chemistry, College of Basic Science, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

    Faezeh Khalilian

Authors
  1. Mohammad Rezaee
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  2. Faezeh Khalilian
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Correspondence to Mohammad Rezaee.

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Mohammad Rezaee declares that she has no conflict of interest. Faezeh Khalilian declares that he has no conflict of interest.

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Rezaee, M., Khalilian, F. Application of Ultrasound-Assisted Extraction Followed by Solid-Phase Extraction Followed by Dispersive Liquid-Liquid Microextraction for the Determination of Chloramphenicol in Chicken Meat. Food Anal. Methods 11, 759–767 (2018). https://doi.org/10.1007/s12161-017-1048-2

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  • DOI: https://doi.org/10.1007/s12161-017-1048-2

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