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Characterization of Separation and Purification Technology and Identification of Taurine from the Bovine Liver

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Abstract

The present study reports an effective method using cation exchange adsorption during the separation and purification of taurine from bovine liver. We use cation exchange method, crystallization and recrystallization, TLC analysis, and infrared spectrometry. The results indicated that the optimum parameters of cation exchange preliminarily separation and purification of taurine are as follows: a 5.66-ml injection volume, a pH value of 4.94, and an elution rate of 2.5 ml/min. Taurine pure product was obtained by crystallization and recrystallization method, its purity more than 98.1%. The results of infrared spectroscopy showed that the purified taurine contain the absorption peak of –NH2 (3212.83, 3045.05, 2971.77 cm−1) and the absorption peak of –SO3H (1184.08, 1305.57, 1511.92, 1585.20, 1616.06 cm−1), consistent with the infrared absorption peaks of taurine standards. The method of separation and purification out of taurine from bovine liver is successful and effectively improves the purity of taurine crude products; it can be applied to industrial production.

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Acknowledgments

This work was supported by the program for China Agriculture Research System (No.CARS-38). We thank the editor and referees for their valuable comments and suggestions on an earlier draft of this manuscript.

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Authors and Affiliations

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China

    Guoyuan Ma, Qunli Yu & Ling Han

  2. Longnan Teachers’ College, Longnan, 732500, China

    Na Sun

  3. Hebei Fucheng Wufeng Food Limited Liability Company, Hebei Province, 065201, China

    Lei Wu

  4. Limited Liability Company of Qin Bao Animal Husbandry in Shanxi Province, Baoji, 721000, China

    Hui Cao

  5. Shandong Lorain Food Limited Company, Shandong Province, Junan County, 276615, China

    Guangxing Han

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  1. Guoyuan Ma
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  2. Na Sun
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Correspondence to Qunli Yu or Ling Han.

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Funding

This work was supported by the China Agriculture Research System (No. CARS-38).

Conflict of Interest

Guoyuan Ma declares that he has no conflict of interest. Na Sun declares that she has no conflict of interest. Qunli Yu declares that he has no conflict of interest. Ling Han declares that she has no conflict of interest. Lei Wu declares that he has no conflict of interest. Hui Cao declares that he has no conflict of interest. Guangxing Han declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the author.

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Ma, G., Sun, N., Yu, Q. et al. Characterization of Separation and Purification Technology and Identification of Taurine from the Bovine Liver. Food Anal. Methods 11, 415–425 (2018). https://doi.org/10.1007/s12161-017-1012-1

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  • DOI: https://doi.org/10.1007/s12161-017-1012-1

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