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Determination of Glucose in Food by the Ionic Liquid and Carbon Nanotubes Modified Dual-Enzymatic Sensors

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Abstract

A sensitive electrochemical glucose biosensor based on chitosan (CS)/glucose oxidase (GOx)/catalase (CAT)/CS + carboxylic multi-walled carbon nanotubes (MWCNTs-COOH) + ionic liquid (IL) film modified glassy carbon electrode has been developed and its characteristics were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Hydrogen peroxide (H2O2) generated during the enzymatic reaction of GOx with glucose could be decomposed by catalase, resulting in the higher sensitivity. The linear range and detection limit were found to be 0.5–100 and 0.2 μmol L−1, respectively. The proposed biosensor was successfully applied for the determination of glucose in drink and food samples with the spiked recoveries in the range of 95.5 to 101.5 %.

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Authors and Affiliations

  1. College of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, 454003, China

    Yunchang Fan, Guitao Hu, Tianao Zhang, Xing Dong, Xiaojing Li, Juan Miao & Shaofeng Hua

  2. Technology Center of Ningbo Entry–Exit Inspection and Quarantine Bureau, Ningbo, 315012, China

    Yingying Zhong

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  1. Yunchang Fan
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  2. Guitao Hu
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  3. Tianao Zhang
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  4. Xing Dong
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  6. Xiaojing Li
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  7. Juan Miao
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  8. Shaofeng Hua
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Correspondence to Juan Miao.

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Conflict of Interest

Yunchang Fan declares that he has no conflict of interest. Guitao Hu declares that he has no conflict of interest. Tianao Zhang declares that he has no conflict of interest. Xing Dong declares that she has no conflict of interest. Yingying Zhong declares that she has no conflict of interest. Xiaojing Li declares that she has no conflict of interest. Juan Miao declares that she has no conflict of interest. Shaofeng Hua declares that he has no conflict of interest.

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This article does not contain any studies with human participants performed by any of the authors.

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Funding

This research was funded by the National Natural Science Foundation of China (grant number 21307028), Basic and Cutting Edge Technology Research Projects of Henan Province (grant numbers 132300410293 and 122300410004), Young Backbone Teachers in Colleges and Universities of Henan Province (grant number 2013GGJS-053), Science and Technology Research Foundation Projects of Henan Province (grant number 142102210049), Natural Science Foundation of the Education Department of Henan Province (grant number 14B150026), and Science Projects of Ningbo City (grant numbers 2012A610149 and 2007C10035).

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Fan, Y., Hu, G., Zhang, T. et al. Determination of Glucose in Food by the Ionic Liquid and Carbon Nanotubes Modified Dual-Enzymatic Sensors. Food Anal. Methods 9, 2491–2500 (2016). https://doi.org/10.1007/s12161-016-0439-0

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  • DOI: https://doi.org/10.1007/s12161-016-0439-0

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