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Effect of Hemicelluloses Fractionated by Graded Ethanol Precipitation from Corn Stover on the Enzymatic Hydrolysis of Lignocellulosic Biomass

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Abstract

The presence of hemicellulose inhibits the enzymatic hydrolysis of lignocellulosic biomass. The purpose of this study is to investigate the effect of different hemicellulose fractions on the enzymatic hydrolysis and the way to eliminate the inhibiting effect caused by hemicellulose. Four kinds of hemicelluloses, namely, HXF, H15, H30, and H60, were first extracted from corn stover by ethanol fractional precipitation. The structures of hemicellulose samples were analyzed using Fourier transform infrared spectroscopy, 1H and 13C nuclear magnetic resonance, and high-performance ion chromatography. The results show that H30 has the strongest inhibition on the enzymatic hydrolysis of Avicel and corn stover, presenting inhibition ratio of 13.35% and 9.98%, respectively. The inhibition ratios of other hemicelluloses in Avicel and corn stover are 8–12% and 5–9%, respectively. However, the inhibiting effect caused by H30 is removed by adding hemicellulase, which even presents a 4.99% increase in the efficiency of enzymatic hydrolysis of corn stover. The corresponding glucose concentration reached 68.11 g/L. This research could help design effective processes to promote the enzymatic hydrolysis of lignocellulosic biomass.

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Funding

This work was financially supported by the National Natural Science Foundation of China (Nos. 22078308 and 22261132515), Young Elite Scientists Sponsorship Program by Henan Association for Science and Technology (No. 2022HYTP018), Innovation Leadership Program in Sciences and Technologies for Central Plains Talent Plan (No. 214200510009), Program for Science & Technology Innovative Research Team in the University of Henan Province (No. 22IRTSTHN007), Key Program of Henan Provincial Science and Technology R&D Plan Joint Fund for Cultivation of Superior Disciplines (No. 222301420008), Innovation Leadership Program in Sciences and Technologies for Zhengzhou Talent Gathering Plan, Innovation Leadership Program in Sciences and Technologies and Local Outstanding Talents for Zhengzhou Talent Gathering Plan (No. 20180400042), Program of Processing and Efficient Utilization of Biomass Resources of Henan Center for Outstanding Overseas Scientists (No. GZS2022007).

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Authors and Affiliations

  1. National Key Laboratory of Biobased Transportation Fuel Technology, School of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, China

    Zhanqiang Yan, Md Asraful Alam, Jinfeng Li, Wenlong Xiong, Shen Zhang, Zili Zhan & Jingliang Xu

  2. Henan Center for Outstanding Overseas Scientists, Zhengzhou, China

    Wenlong Xiong & Jingliang Xu

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  1. Zhanqiang Yan
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Conceptualization: Jingliang Xu, Wenlong Xiong; methodology: Jingliang Xu, Wenlong Xiong, Zhanqiang Yan; investigation: Jingliang Xu, Wenlong Xiong, Zhanqiang Yan, Md Asraful Alam; supervision: Jingliang Xu, Zili Zhan; writing—original draft: Zhanqiang Yan, Jinfeng Li; writing—review and editing: Jingliang Xu, Wenlong Xiong, Md Asraful Alam, Shen Zhang; funding: Jingliang Xu, Wenlong Xiong.

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Correspondence to Wenlong Xiong or Jingliang Xu.

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Yan, Z., Alam, M.A., Li, J. et al. Effect of Hemicelluloses Fractionated by Graded Ethanol Precipitation from Corn Stover on the Enzymatic Hydrolysis of Lignocellulosic Biomass. Bioenerg. Res. (2024). https://doi.org/10.1007/s12155-024-10745-4

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