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Flow configuration and Packing Density of Sugarcane Bagasse and Wheat Bran Affecting Enzymes Production by Solid-State Fermentation

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Abstract

Cellulolytic and hemicellulolytic enzymes are essential to make feasible the fully biochemical route for second-generation ethanol production. Solid-state fermentation figures as a low-cost and sustainable way of obtaining such enzymes by using agro-industrial by-products as substrates. In the current paper, thermal conditions and enzymatic activities from the cultivations of Myceliophthora thermophila, Trichoderma reesei, and Aspergillus niger were evaluated. Mixtures of sugarcane bagasse (SCB) as fibers or powder and wheat bran (WB) were used as substrates within a packed-bed bioreactor with variable packing density, as well as under two fluid flow configurations. Using SCB as fibers and WB 7:3 w/w (packing density 0.1 g/cm3) for M. thermophila, endoglucanase and xylanase activities overcame 850 and 3850 U/g, with temperature rise < 1 °C. Cultivations of M. thermophila and T. reesei in pure WB or with SCB as powder led to temperature rises of 7 to 16 °C, due to lower bed porosity. A. niger cultivations provided endoglucanase activities around 15 U/g under 8 ºC of maximum temperature rise, with no difference among percolating air and jacket water flowing in co- or counter current. The reported results address the importance of a strategic definition of the porous media composition and packing technique for a successful scale-up of the bioprocess, while giving operational flexibility in terms of fluid flow configuration.

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Funding

The authors gratefully acknowledge the funding of this work by the São Paulo Research Foundation (FAPESP) (grants #2018/16689–1; #2018/00996–2; #2016/10636–8), the Brazilian National Council for Scientific and Technological Development (CNPq), grants #2018/430786–2; #448890/2014–3), and the Coordination for the Improvement of Higher Education Personnel (CAPES, Finance Code 001).

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Authors and Affiliations

  1. Graduate Program of Chemical Engineering, Department of Chemical Engineering, Center of Exact Sciences and of Technology, Federal University of São Carlos (UFSCar), Rod. Washington Luiz Km 235, São Carlos-SP, SP-310, 13565-905, Brazil

    Fernanda Perpétua Casciatori, Nilton Silva Costa Mafra, Natalia Alvarez Rodrigues & Everton Holanda Sales

  2. Graduate Program of Digital Agroenergy, Federal University of Tocantins (UFT), Quadra 109 Norte Avenida NS 15, Palmas-TO, 77001-090, Brazil

    Mariana Moreira Sidel Maia, Ana Caroline Pereira Dias & Lina María Grajales

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  1. Fernanda Perpétua Casciatori
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and experiments were performed by Fernanda Perpétua Casciatori, Nilton Silva Costa Mafra, Éverton Holanda Sales, and Natalia Alvarez Rodrigues. Processing of figures and graphics and analysis of the results were done by the four authors just mentioned plus Mariana Moreira Sidel Maia, Ana Caroline Pereira Dias, and Lina María Grajales. The first draft of the manuscript was written by Fernanda Perpétua Casciatori, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript for submission, as first version and after major revision.

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Correspondence to Lina María Grajales.

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Casciatori, F.P., Mafra, N.S.C., Rodrigues, N.A. et al. Flow configuration and Packing Density of Sugarcane Bagasse and Wheat Bran Affecting Enzymes Production by Solid-State Fermentation. Bioenerg. Res. 17, 187–197 (2024). https://doi.org/10.1007/s12155-023-10675-7

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