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Biomass and Lipid Productivities of Cyanobacteria- Leptolyngbya foveolarum HNBGU001

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Abstract

The initial biomass density had a significant effect on the growth behaviour of Leptolyngbya foveolarum HNBGU-001 in the batch culture. The test organism produced substantial amounts of biomass and lipids when allowed to grow at 40 °C and pH 8.0. The Placket-Burman design appropriately indicated the positive and negative influences of the tested nutrient factors on biomass and lipid productivities of L. foveolarum. Employing the Box-Behnken design of response surface methodology, the elevated concentrations of sulphate-S (300 mg/L) and carbonate-C (45.39 mg/L) but relatively low levels of phosphate-P (10 mg/L) and nitrate-N (375 mg/L) were identified as the optimal nutritional requirements of the test organism for the maximum lipid productivity (49.60 ± 0.70 mg/L/day) along with high lipid content (32.10 ± 0.40% dry cell weight) and biomass productivity (154.80 ± 3.60 mg/L/day). These maximized responses of biomass productivity, lipid content, and lipid productivity were respectively 2.8-, 2.4-, and 6.8-fold higher than their corresponding values under the unoptimized conditions. FAME analysis of lipids obtained from the test organism revealed some promising features for their use as algal biodiesel feedstock. Nonetheless, the test organism seems to have better potential than several other microalgae for use in biofuel production as it can give a high output of biomass as also lipids at elevated temperature prevalent in outdoor conditions of subtropical and tropical regions.

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All the data are available with the corresponding author and may be provided on request.

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Acknowledgements

The authors are thankful to the Head, Department of Botany and Microbiology, H.N.B. Garhwal University, for providing necessary facilities. PS thanks the University Grants Commission, New Delhi, for financial assistance in the form of a University Research Fellowship and CSIR-Indian Institute of Petroleum, Dehradun, for GC-MS analysis.

Funding

1. University Grants Commission, New Delhi, India

2. Department of Botany and Microbiology, Hemvati Nandan Bahuguna Garhwal University, Srinagar Garhwal, India

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  1. Laboratory of Algal Biotechnology, Department of Botany and Microbiology, School of Life Sciences, H.N.B. Garhwal University, Srinagar, Garhwal, 246 174, India

    Preeti Singh & Dhananjay Kumar

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PS planned and executed experiments, prepared a draft of the manuscript, and finalized the manuscript for submission. D. Kumar was involved in conceiving the idea and planning of the experiments. Being the supervisor of the first and corresponding author, he was responsible for the organization of lab and experimental facilities for the work contained in the paper.

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Correspondence to Preeti Singh.

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Supplementary Fig. 1

Perturbation plots of factors influencing biomass productivity (a) and lipid content (b) of L. foveolorum HNBGU-001 [A: nitrate-N, B: phosphate-P, C: sulphate-S, D: carbonate-C, E: Ca2+, F: Fe3+, K: Dummy factor]. (PNG 344 kb).

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Singh, P., Kumar, D. Biomass and Lipid Productivities of Cyanobacteria- Leptolyngbya foveolarum HNBGU001. Bioenerg. Res. 14, 278–291 (2021). https://doi.org/10.1007/s12155-020-10170-3

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