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Backbone and side chain NMR assignments of the H-NOX domain from Nostoc sp. in complex with BAY58-2667 (cinaciguat)

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A Correction to this article was published on 05 January 2021

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Abstract

Soluble guanylate cyclase (sGC) enzyme is activated by the gaseous signaling agent nitric oxide (NO) and triggers the conversion of GTP (guanosine 5′-triphosphate) to cGMP (cyclic guanylyl monophosphate). It contains the heme binding H-NOX (heme-nitric oxide/oxygen binding) domain which serves as the sensor of NO and it is highly conserved across eukaryotes and bacteria as well. Many research studies focus on the synthesis of chemical compounds bearing possible therapeutic action, which mimic the heme moiety and activate the sGC enzyme. In this study, we report a preliminary solution NMR (Nuclear Magnetic Resonance) study of the H-NOX domain from Nostoc sp. cyanobacterium in complex with the chemical sGC activator cinaciguat (BAY58-2667). An almost complete sequence-specific assignment of its 1H, 15N and 13C resonances was obtained and its secondary structure predicted by TALOS+.

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Assignment deposited in BMRB with ID: 28,118.

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Author notes

  1. Garyfallia I. Makrynitsa and Aikaterini I. Argyriou have equally contributed to this work.

Authors and Affiliations

  1. Department of Pharmacy, University of Patras, 26504, Patras, Greece

    Garyfallia I. Makrynitsa, Aikaterini I. Argyriou, Georgios Dalkas, Dimitra A. Georgopoulou & Georgios A. Spyroulias

  2. Institut für Organische Chemie, Universität Leipzig, Johannisallee 29, 04103, Leipzig, Germany

    Marina Bantzi & Athanassios Giannis

  3. Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700, Fribourg, Switzerland

    Marina Bantzi

  4. Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece

    Andreas Papapetropoulos

Authors
  1. Garyfallia I. Makrynitsa
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  2. Aikaterini I. Argyriou
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  3. Georgios Dalkas
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  4. Dimitra A. Georgopoulou
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  5. Marina Bantzi
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  6. Athanassios Giannis
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  7. Andreas Papapetropoulos
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  8. Georgios A. Spyroulias
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Contributions

Garyfallia I. Makrynitsa and Aikaterini I. Argyriou have equally contributed to this work.

Corresponding author

Correspondence to Georgios A. Spyroulias.

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Makrynitsa, G.I., Argyriou, A.I., Dalkas, G. et al. Backbone and side chain NMR assignments of the H-NOX domain from Nostoc sp. in complex with BAY58-2667 (cinaciguat). Biomol NMR Assign 15, 53–57 (2021). https://doi.org/10.1007/s12104-020-09982-3

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  • DOI: https://doi.org/10.1007/s12104-020-09982-3

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