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Backbone 1H, 13C, and 15N NMR resonance assignments of the Krüppel-like factor 4 activation domain

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Abstract

Krüppel-like factor 4 (KLF4) is a transcription factor involved in diverse biological processes, including development, cellular differentiation and proliferation, and maintenance of tissue homeostasis. KLF4 has also been associated with disease states, such as cardiovascular disease and several cancers. KLF4 contains an activation domain, repression domain, and a structurally characterized C-terminal zinc finger domain that mediates its binding to DNA. The structurally uncharacterized KLF4 activation domain is critical for transactivation by KLF4 and mediates its binding to the transcriptional coactivator CBP/p300. Here, we report the 1H, 15N, 13CO, 13Cα and 13Cβ NMR chemical shift assignments of KLF41–130, which contains the KLF4 activation domain. Narrow chemical shift dispersion in the 1H dimension of the 1H-15N HSQC spectrum suggests that the KLF41–130 fragment is intrinsically disordered.

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Acknowledgements

This work was funded by a Natural Science and Engineering Council of Canada discovery grant to SPS. DNL is a recipient of a CIHR Postdoctoral Fellowship and BSC was supported by a Queen’s University Chancellor’s Scholarship.

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Authors and Affiliations

  1. Department of Biomedical & Molecular Sciences, Queen’s University, Kingston, ON, Canada, K7L 3N6

    Brigid S. Conroy, Emma R. Weiss & Steven P. Smith

  2. Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2

    David N. Langelaan

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  1. Brigid S. Conroy
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  2. Emma R. Weiss
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  3. Steven P. Smith
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  4. David N. Langelaan
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Correspondence to Steven P. Smith or David N. Langelaan.

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Conroy, B.S., Weiss, E.R., Smith, S.P. et al. Backbone 1H, 13C, and 15N NMR resonance assignments of the Krüppel-like factor 4 activation domain. Biomol NMR Assign 11, 95–98 (2017). https://doi.org/10.1007/s12104-017-9727-x

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