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Backbone resonance assignments for the PHD-Bromo dual-domain of the human chromatin reader TRIM24

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Abstract

Plant homeodomains (PHD) and Bromo domains are both chromatin reader domains that recognise histone methylation degree and acetylation state, respectively. The tripartite motif protein TRIM24 is a multidomain protein carrying a PHD-Bromo motif at its C-terminus, through which it is able to bind to histone 3 (H3) N-terminal tails with a specific modification pattern, namely unmethylated at K4 and acetylated at K23 (H3-K4me0K23ac). Here we report the \({}^{1}\hbox {H}, {}^{13}\hbox {C}\) and \({}^{15}\hbox {N}\) backbone resonance assignment of this 23 kDa motif, which we have obtained by heteronuclear multidimensional NMR spectroscopy. Furthermore we show that the secondary \(\hbox {C}_\alpha\) and \(\hbox {C}_\beta\) chemical shifts are in good agreement with a previously published crystal structure.

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References

  • Allton K, Jain AK, Herz HM, Tsai WW, Jung SY, Qin J, Bergmann A, Johnson RL, Barton MC (2009) TRIM24 targets endogenous p53 for degradation. Proc Natl Acad Sci USA 106(28):11,612–11,616

    Article  Google Scholar 

  • Chen C, Seth AK, Aplin AE (2006) Genetic and expression aberrations of E3 ubiquitin ligases in human breast cancer. Mol Cancer Res 4(10):695–707

    Article  Google Scholar 

  • Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293

    Article  Google Scholar 

  • Grzesiek S, Bax A (1992a) An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins. J Magn Reson 99:201–207

    ADS  Google Scholar 

  • Grzesiek S, Bax A (1992b) Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR. J Am Chem Soc 114:6291–6293

    Article  Google Scholar 

  • Kay LE, Ikura M, Tschudin R, Bax A (1990) Three-dimensional triple-resonance NMR spectroscopy of isotopically enriched proteins. J Magn Reson 89:496–514

    ADS  Google Scholar 

  • Keller RLJ (2004) The computer aided resonance tutorial. Cantina, Goldau

    Google Scholar 

  • Le Douarin B, Zechel C, Garnier JM, Lutz Y, Tora L, Pierrat P, Heery D, Gronemeyer H, Chambon P, Losson R (1995) The N-terminal part of TIF1, a putative mediator of the ligand-dependent activation function (AF-2) of nuclear receptors, is fused to B-raf in the oncogenic protein T18. EMBO J 14(9):2020–2033

    Google Scholar 

  • Le Douarin B, Nielsen AL, Garnier JM, Ichinose H, Jeanmougin F, Losson R, Chambon P (1996) A possible involvement of TIF1 alpha and TIF1 beta in the epigenetic control of transcription by nuclear receptors. EMBO J 15(23):6701–6715

    Google Scholar 

  • Live DH, Davis DG, Agosta WC, Cowburn D (1984) Observation of 1000-fold enhancement of nitrogen-15 NMR via proton-detected multiquantum coherences: studies of large peptides. J Am Chem Soc 106(20):6104–6105

    Article  Google Scholar 

  • Reddy BA, Etkin LD, Freemont PS (1992) A novel zinc finger coiled-coil domain in a family of nuclear proteins. Trends Biochem Sci 17(9):344–345

    Article  Google Scholar 

  • Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (2001) The tripartite motif family identifies cell compartments. EMBO J 20(9):2140–2151

    Article  Google Scholar 

  • Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223

    Article  Google Scholar 

  • Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, Patel DJ, Barton MC (2010) TRIM24 links a non-canonical histone signature to breast cancer. Nature 468(7326):927–932

    Article  ADS  Google Scholar 

  • Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4(2):171–180

    Article  Google Scholar 

Download references

Acknowledgments

We would like to thank Liz Flavell for establishing the protein expression and purification protocols.

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Authors and Affiliations

  1. Structure and Biophysics, AstraZeneca Discovery Sciences, Alderley Park, SK10 4TG, United Kingdom

    Reto Walser & Alexander G. Milbradt

  2. RAD, AstraZeneca Discovery Sciences, Alderley Park, SK10 4TG, United Kingdom

    Jonathan Renshaw

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  1. Reto Walser
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  2. Jonathan Renshaw
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  3. Alexander G. Milbradt
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Correspondence to Reto Walser.

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Walser, R., Renshaw, J. & Milbradt, A.G. Backbone resonance assignments for the PHD-Bromo dual-domain of the human chromatin reader TRIM24. Biomol NMR Assign 10, 207–211 (2016). https://doi.org/10.1007/s12104-016-9668-9

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  • DOI: https://doi.org/10.1007/s12104-016-9668-9

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