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The expression and function of Frizzled-7 in human renal cell carcinoma

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Abstract

Purpose

Wnt/β-catenin has emerged as an important signal pathway in renal cell carcinoma (RCC) pathogenesis. Frizzled 7 (Fzd7) is a member of Frizzled (Fzd) receptor family which binds with Wnt ligands and transduces canonical and non-canonical pathways. However, the expression of Fzd7 in human RCC is poorly investigated.

Methods

53 RCC tissues and peri-tumor tissues were collected from the patients treated with radical nephrectomy. The expression of Fzd7 was investigated by immunohistochemical staining. Three RCC cells were transfected with Fzd7shRNA and GFPshRNA to investigate the function of Fzd7 in RCC cells.

Results

The immunohistochemical analysis showed that Fzd7 protein expression level was significantly increased in RCC tissues when compared with peri-tumor tissues, which suggested that Fzd7 might be involved in the formation of tumors. However, the Fzd7 expression was not correlated with clinicopathological parameters. Three RCC cell lines: 786-O, Caki-1, and OS-RC-2 also expressed Fzd7. With Fzd7 expression being interfered by shRNA, the RCC cell proliferation was mildly decreased. Wnt3a could stimulate the RCC cells proliferation, but the stimulation was decreased when Fzd7 expression was interfered. Restoring the Fzd7 expression led to the proliferation stimulation effect of Wnt3a being restored.

Conclusions

This paper suggests that Fzd7 may act as one of the molecules that take part in the course of RCC formation. Fzd7 can be activated by Wnt3a to stimulate cell proliferation.

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Acknowledgments

This work was supported by National Nature Science Foundation of China (Grant No. 31370872) and National Undergraduates Innovating Experimentation Project (Grant No. 201310285048Z).

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Authors and Affiliations

  1. Department of Pharmacy, College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China

    R. Xu, S. Zeng, Y. L. Chen, M. J. Chen & L. Zhang

  2. Department of Immunology, School of Basic Medicine and Life Sciences, Medical College, Soochow University, Suzhou, China

    W. Xie

  3. The Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, China

    C. Sun

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  1. R. Xu
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  2. S. Zeng
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  3. W. Xie
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  5. Y. L. Chen
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  6. M. J. Chen
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  7. L. Zhang
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Correspondence to L. Zhang.

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Ethics approval has been obtained from the Institution Review Board (IRB) committee of Soochow University.

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Xu, R., Zeng, S., Xie, W. et al. The expression and function of Frizzled-7 in human renal cell carcinoma. Clin Transl Oncol 18, 269–276 (2016). https://doi.org/10.1007/s12094-015-1362-3

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  • DOI: https://doi.org/10.1007/s12094-015-1362-3

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