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FBXW7 overexpression suppresses renal cancer cell proliferation and induces apoptosis

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Abstract

FBXW7 gene (F-box and WD-40 domain protein 7) is also named HCDC4 and is a significant tumor suppressor gene, which can regulate human cell cycle, proliferation and differentiation. In this study, we tend to investigate protein expression and related biological functions of FBXW7 gene. FBXW7 expression level in renal cell carcinoma (RCC) tissues is highly related to its clinical pathologic grade (P = 0.0094) and TNM phase (P = 0.0080) and is highly lower than in paracancerous normal tissues through immunohistochemistry study. FBXW7 high-expression patients have overall better prognosis than low-expression patients (P < 0.001). After transfected with FBXW7 plasmid, the RCC cell lines ACHN and A704 showed a depressed proliferation activity and high proportion of apoptosis through CCK8, colony formation and flow cytometry assay studies. By Western blot analysis, expression of cell proliferation-activating protein c-Myc and c-Jun is downregulated in FBXW7 high-expression RCC compared with negative control. These data suggested that FBXW7 is a significant tumor suppressor gene in RCC.

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    Authors and Affiliations

    1. Department of Urology, University of South China of Collaboration Hospital, Guangdong No. 2 Provincial People’s Hospital, Guangzhou, 510317, Guangdong Province, China

      Yu Fu, Guosheng Yang, Gaoyuan Li, Yuejia Liu, Jun Zeng, Yangke Cai & Jian Bai

    2. Pathology Department, University of South China of Collaboration Hospital, Guangdong No. 2 Provincial People’s Hospital, Guangzhou, 510095, Guangdong Province, China

      Xi Tan

    3. Southern Medical University, Guangzhou, 510280, Guangdong Province, China

      Youcheng Lin, Xun Wu & Yukun Ge

    4. University of Chinese Academy of Sciences, Beijing, 100049, China

      Zhao Yang

    5. National-Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, China

      Yi Huang, Yongqiang Wang, Meng Zhang, Lu Fang & Song Wu

    6. Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China

      Song Wu

    7. Luohu Hospital of Shenzhen University, Shenzhen Fifth People’s Hospital, Shenzhen, 518035, China

      Song Wu

    8. The Second Hospital of Lanzhou University, Xian, 710000, Shanxi Province, China

      Hu Xiong

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    1. Yu Fu
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    15. Jun Zeng
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    Corresponding authors

    Correspondence to Guosheng Yang or Song Wu.

    Additional information

    Yu Fu, Youcheng Lin, Zhao Yang and Guosheng Yang have contributed equally to this work.

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    Cite this article

    Fu, Y., Lin, Y., Yang, Z. et al. FBXW7 overexpression suppresses renal cancer cell proliferation and induces apoptosis. Med Oncol 32, 215 (2015). https://doi.org/10.1007/s12032-015-0656-1

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