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Metagenomic Analysis Reveals the Complex Microbial Landscape of Market Chicken Meat

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Abstract

The safety of meat from a microbiological standpoint is of paramount concern to public health, given the potential for bacterial contaminants to grow and persist during processing and storage. To address this issue, a culture-independent approach targeting the V3-V4 region of the 16S rRNA gene was utilized to investigate the inherent bacterial communities present in 10 chicken meat samples obtained from retail markets. Amplicons were sequenced using the Illumina MiSeq platform, and unique amplicon sequence variants (ASVs) were identified using the DADA2 pipeline. Results indicated the presence of 5 phyla, 7 classes, 16 orders, 33 families, 59 genera, and 273 unique ASVs. The dominant families were Flavobacteriaceae, Moraxellaceae, Enterobacteriaceae, Wohlfahrtiimonadaceae, Morganellaceae, and Pseudomonadaceae, comprising 27.03, 22.04, 15.67, 9.40, 7.92, and 5.02% of the identified families, respectively. Functional analysis using PICRUSt showed a diverse range of functional pathways. These findings have significant implications for policymaking regarding food safety and public health. Regular monitoring of bacterial communities in meat products is crucial to ensure their safety for consumption. This study demonstrates the utility of culture-independent approaches in characterizing microbial communities, which can provide valuable information for ensuring food safety and safeguarding public health.

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Acknowledgements

The authors are thankful to the Principal, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand for providing all the necessary facilities and consumables, Department of Animal Biotechnology, Anand, for providing the platform to carry out the work.

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Authors and Affiliations

  1. Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat, 388001, India

    Pranav Anjaria, Manasi Soni, Jitendrakumar Nayak & M. N. Brahmbhatt

  2. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat, 388001, India

    Prakash Koringa & Mansi Desai

  3. Department of Livestock Products Technology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat, 388001, India

    Prakrutik Bhavsar

  4. Molecular Biology and Virology, HiMedia Laboratories Pvt. Ltd., Thane, 400604, India

    Somak Chowdhury & Kamlesh Jangid

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  1. Pranav Anjaria
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Correspondence to M. N. Brahmbhatt.

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Anjaria, P., Koringa, P., Bhavsar, P. et al. Metagenomic Analysis Reveals the Complex Microbial Landscape of Market Chicken Meat. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-024-01249-y

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