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Degradation of Nitriles by Mixed Biofilms of Nitrile-Hydrolyzing Bacteria in Submerged Packed-Bed Reactor

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Abstract

Degradation of nitriles by mixed biofilms of nitrile-hydrolyzing bacteria Alcaligenes faecalis 2 and Rhodococcus ruber gt 1 grown on basalt and carbon carriers, in a submerged packed-bed reactor was studied. It was shown the formation of a massive mixed biofilm of Al. faecalis 2 and R. ruber gt 1 and the effective removal of nitriles and products of their degradation from the reaction medium. After the accumulation of carboxylic acid and some of the unprocessed substrate, the system adapts to 600–1000 h of biofilter operation, which is expressed in a decrease in the content of substrate and reaction products in the medium. The rate of acetonitrile and acrylonitrile utilization was 0.072–0.086 and 0.039–0.215 g/h, respectively, and acrylonitrile utilization with maximum rate was realized by a mixed biofilm on carbon fibers. Biofilms grown on mixed fibers in a "sandwich"-type reactor had the best characteristics for the transformation of aceto- and acrylonitrile (removal capacity of 99.6–99.9%, nitrile utilization rate of 0.080–0.095 g/h). Biofilms grown on basalt fiber with a diameter of 4–12 μm are also well suited for the degradation of acetonitrile (removal capacity of 100%, nitrile utilization rate of 0.086 g/h). The results of metagenomic analysis showed the resistance of Al. faecalis 2 and R. ruber gt 1 mixed biofilms against leaching from a biofilter and to competitive growth in an open system, indicating the advantages of biofilms over homogeneous biomass for wastewater treatment from nitrile compounds. Biofilms of two species of nitrile hydrolyzing bacteria on basalt and carbon fibers effectively purify water from nitriles in a submerged packed-bed reactor.

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References

  1. Debabov VG, Yanenko AS (2011) Biocatalytic hydrolysis of nitriles. Obz Zh Khim 1(4):376–394. https://doi.org/10.1134/S2079978011030010

    Article  Google Scholar 

  2. Achanzar WE, Mangipudy RS (2014) Acrylonitrile. In: Wexler P (ed) Encyclopedia of toxicology, 3rd edn. Academic Press, London, pp 76–78

    Chapter  Google Scholar 

  3. Caito S, Costa LG, Rongzhu L, Aschner M (2014) Acrylonitrile. In: Aminoff MJ, Daroff RB (eds) Encyclopedia of the neurological sciences, 2nd edn. Academic Press, London, pp 33–35

    Chapter  Google Scholar 

  4. Liu Q, Fang B, Bai X, Liu Y, Wu Y, Xu G, Guo C (2016) Direct synthesis of nitriles from cleavage of C=C double bond with nitrite as the nitrogen source and oxidant. Tetrahedron lett 57(24):2620–2623. https://doi.org/10.1016/j.tetlet.2016.04.108

    Article  CAS  Google Scholar 

  5. Robles H (2014) Acetonitrile. In: Wexler P (ed) Encyclopedia of toxicology, 3rd edn. Academic Press, London, pp 40–42

    Chapter  Google Scholar 

  6. Zhai Y, St-Pierre J (2019) Acetonitrile contamination in the cathode of proton exchange membrane fuel cells and cell performance recovery. Appl Energy 242:239–247. https://doi.org/10.1016/j.apenergy.2019.03.086

    Article  CAS  Google Scholar 

  7. Zhan W, Tong M, Ji L, Zhang H, Ge Z, Wang X, Li R (2019) Continuous-flow synthesis of nitriles from aldehydes via Schmidt reaction. Chinese Chem Lett 30(5):973–976. https://doi.org/10.1016/j.cclet.2019.01.006

    Article  CAS  Google Scholar 

  8. Zheng L, Pan L, Miao J, Lin Y, Wu J (2018) Application of a series of biomarkers in Scallop Chlamys farreri to assess the toxic effects after exposure to a priority hazardous and noxious substance (HNS) – Acrylonitrile. Environ Toxicol Pharm 64:122–130. https://doi.org/10.1016/j.etap.2018.10.002

    Article  CAS  Google Scholar 

  9. Gupta N, Balomajumber Ch, Agarwal VK (2010) Enzymatic mechanism and biochemistry for cyanide degradation: a review. J Hazard Mater 176:1–13. https://doi.org/10.1016/j.jhazmat.2009.11.038

    Article  PubMed  CAS  Google Scholar 

  10. Prasad S, Bhalla TC (2010) Nitrile hydratases (NHases): At the interface of academia and industry. Biotechnol Adv 28:725–741. https://doi.org/10.1016/j.biotechadv.2010.05.020

    Article  PubMed  CAS  Google Scholar 

  11. Kohyama E, Yoshimura A, Aoshima D, Yoshida T, Kawamoto H, Nagasawa T (2006) Convenient treatment of acetonitrile-containing wastes using the tandem combination of nitrile hydratase and amidase-producing microorganisms. Appl Microbiol Biotechnol 72:600–606. https://doi.org/10.1007/s00253-005-0298-x

    Article  PubMed  CAS  Google Scholar 

  12. Kohyama E, Dohi M, Yoshimura A, Yoshida T, Nagasawa T (2007) Remaining acetamide in acetonitrile degradation using nitrile hydratase- and amidase-producing microorganisms. Appl Microbiol Biotechnol 74:829–835. https://doi.org/10.1007/s00253-006-0738-2

    Article  PubMed  CAS  Google Scholar 

  13. Chen CY, Chen SC, Fingas M, Kao CM (2010) Biodegradation of propionitrile by Klebsiella oxytoca immobilized in alginate and cellulose triacetate gel. J Hazard Mater 177:856–863. https://doi.org/10.1016/j.jhazmat.2009.12.112

    Article  PubMed  CAS  Google Scholar 

  14. Li C, Sun Y, Yue Z, Huang M, Wang J, Chen X, An X, Zang H, Li D, Hou N (2018) Combination of a recombinant bacterium with organonitrile-degrading and biofilm-forming capability and a positively charged carrier for organonitriles removal. J Hazard Mater 353:372–380. https://doi.org/10.1016/j.jhazmat.2018.03.058

    Article  PubMed  CAS  Google Scholar 

  15. An X, Cheng Y, Huang M, Sun Y, Wang H, Chen X, Wang J, Li D, Li C (2018) Treating organic cyanide-containing groundwater by immobilization of a nitrile-degrading bacterium with a biofilm-forming bacterium using fluidized bed reactors. Environ Pollut 237:908–916. https://doi.org/10.1016/j.envpol.2018.01.087

    Article  PubMed  CAS  Google Scholar 

  16. Li T, Bai R, Ohandja D-G, Liu J (2009) Biodegradation of acetonitrile by adapted biofilm in a membrane-aerated biofilm reactor. Biodegradation 20(4):569–580. https://doi.org/10.1007/s10532-008-9246-7

    Article  PubMed  CAS  Google Scholar 

  17. Demakov VA, Vasil’ev DM, Maksimova YG, Pavlova YA, Ovechkina GV, Maksimov AY (2015) Activated sludge bacteria transforming cyanopyridines and amides of pyridinecarboxylic acids. Microbiol 84:433–441. https://doi.org/10.1134/S0026261715030030

    Article  CAS  Google Scholar 

  18. Maksimov AY, Kuznetsova MV, Ovechkina GV, Kozlov SV, Maksimova YG, Demakov VA (2003) Effects of nitriles and amides on the growth and nitrile hydratase activity of the Rhodococcus sp. strain gt1. Appl Biochem Microbiol 39(1):55–59. https://doi.org/10.1023/A:1021798010261

    Article  CAS  Google Scholar 

  19. Zorina AS, Maksimova YG, Demakov VA (2019) Biofilm formation by monocultures and mixed cultures of Alcaligenes faecalis 2 and Rhodococcus ruber gt 1. Microbiol 88(2):164–171. https://doi.org/10.1134/S0026261719020140

    Article  CAS  Google Scholar 

  20. Maksimova YG, Vasil’ev DM, Zorina AS, Ovechkina GV, Maksimov AY (2018) Acrylamide and acrylic acid biodegradation by Alcaligenes faecalis 2 planktonic cells and biofilms. Appl Biochem Microbiol 54(2):173–178. https://doi.org/10.1134/S0003683818020084

    Article  CAS  Google Scholar 

  21. Manolov T, Håkansson K, Benoit G (2005) Continuous acetonitrile degradation in a packed-bed bioreactor. Appl Microbiol Biotechnol 66:567–574. https://doi.org/10.1007/s00253-004-1744-x

    Article  PubMed  CAS  Google Scholar 

  22. Li C, Yue Z, Feng F, Xi C, Zang H, An X, Liu K (2016) A novel strategy for acetonitrile wastewater treatment by using a recombinant bacterium with biofilm-forming and nitrile-degrading capability. Chemosphere 161:224–232. https://doi.org/10.1016/j.chemosphere.2016.07.019

    Article  PubMed  CAS  Google Scholar 

  23. Dash RR, Gaur A, Balomajumder C (2009) Cyanide in industrial wastewaters and its removal: a review on biotreatment. J Hazard Mater 163:1–11. https://doi.org/10.1016/j.jhazmat.2008.06.051

    Article  PubMed  CAS  Google Scholar 

  24. An X, Cheng Y, Miao L, Chen X, Zang H, Li C (2020) Characterization and genome functional analysis of an efficient nitrile-degrading bacterium, Rhodococcus rhodochrous BX2, to lay the foundation for potential bioaugmentation for remediation of nitrile-contaminated environments. J Hazard Mater 389:121906. https://doi.org/10.1016/j.jhazmat.2019.121906

    Article  PubMed  CAS  Google Scholar 

  25. Alvillo-Rivera A, Garrido-Hoyos S, Buitrón G, Thangarasu-Sarasvathi P, Rosano-Ortega G (2021) Biological treatment for the degradation of cyanide: a review. J Mater Res Technol 12:1418–1433. https://doi.org/10.1016/j.jmrt.2021.03.030

    Article  CAS  Google Scholar 

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Acknowledgements

The work was carried out within the framework of the state assignment on the topic " Search and selection of new promising microorganisms for the purposes of biotechnology. Creation of immunochemical diagnostic systems ", registration number R&D 122010800029-1.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia, 614081

    A. S. Zorina, A. Yu. Maksimov & Yu. G. Maksimova

  2. Perm State National Research University, Perm, Russia, 614990

    A. Yu. Maksimov & Yu. G. Maksimova

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  1. A. S. Zorina
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  3. Yu. G. Maksimova
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Correspondence to A. S. Zorina.

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Zorina, A.S., Maksimov, A.Y. & Maksimova, Y.G. Degradation of Nitriles by Mixed Biofilms of Nitrile-Hydrolyzing Bacteria in Submerged Packed-Bed Reactor. Indian J Microbiol 62, 610–617 (2022). https://doi.org/10.1007/s12088-022-01030-z

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