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Fermentative Production of Pyranone Derivate I from Marine Vibrio sp. SKMARSP9: Isolation, Characterization and Bioactivity Evaluation

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Abstract

Pyranone derivative I was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. SKMARSP9. The compound I was characterized, and evaluated for its antimicrobial properties. The isolated strain was identified based on 16S rRNA based phylogenetic analysis. The molecular analysis data suggested that this strain is closely related to Vibrio ruber, Vibrio sp. MSSRF10 and Vibrio rhizosphaerae. The best fermentative growth of this isolate was achieved under halophilic conditions and grew efficiently at 30 °C in the presence of 12 % NaCl. The compound I production by this strain is associated with growth. The unpurified extract is hydrophobic in nature, and released only during late growth phase. The extract was purified and characterized by spectral data using NMR, DEPT, and ESI–MS. The purity of I was 97 % which was confirmed by HPLC. The pyranone derivative I exhibited >50 % antioxidant activity and broad spectrum antimicrobial properties against gram negative and gram positive strains. Molecular docking analysis revealed that this pyranone derivative I may be a potential candidate at pharmaceutical sector.

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Acknowledgments

The authors B. Sudheer Kumar and P. Raju were thankful to CSIR for financial support as Senior Research Fellowship. The authors were thankful to Ingo Morgenstern, Centre for Structural Functional Genomics, Concordia University.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Microbiology, Kakatiya University, Warangal, 506009, India

    P. Shiva Krishna & M. A. Singara Charya

  2. Medicinal Chemistry & Pharmacology, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007, India

    P. Raju & M. S. R. Murty

  3. Centre for NMR & Structural Chemistry, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007, India

    T. Prabhakar Rao

  4. Bio Engineering & Environmental Sciences, CSIR - Indian Institute of Chemical Technology, Hyderabad, 500007, India

    B. Sudheer Kumar & R. S. Prakasham

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  1. P. Shiva Krishna
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  4. M. S. R. Murty
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  6. M. A. Singara Charya
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  7. R. S. Prakasham
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Correspondence to R. S. Prakasham.

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Shiva Krishna, P., Sudheer Kumar, B., Raju, P. et al. Fermentative Production of Pyranone Derivate I from Marine Vibrio sp. SKMARSP9: Isolation, Characterization and Bioactivity Evaluation. Indian J Microbiol 55, 292–301 (2015). https://doi.org/10.1007/s12088-015-0521-0

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  • DOI: https://doi.org/10.1007/s12088-015-0521-0

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