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Development of two-dimensional NMR

Structure determination of biomolecules in solution

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Abstract

The development of Fourier transform NMR in the mid 1960’s, did parallel processing of the collection of NMR data, increased the signal/noise ratio by two orders of magnitude and made it possible to record the proton NMR spectra of small proteins which contain hundreds of resonances. The assignment of these resonances then became a challenge, which was solved with the development of two-dimensional Fourier transform NMR, also called 2D NMR. This article describes the initial start and development of the various methods of 2D NMR leading to structure determination of proteins in solution and two Nobel Prizes.

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Authors and Affiliations

  1. Department of Physics and NMR Research Centre, Indian Institute of Science, Bengaluru, 560 012, India

    Anil Kumar

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  1. Anil Kumar
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Correspondence to Anil Kumar.

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Anil Kumar, Department of Physics and NMR Research Centre, IISc, Bengaluru, is currently National Academy of Sciences India (NASI) Senior Scientist Platinum Jubilee Fellow. His area of research is ‘NMR technique development’ with applications to biomolecular structure determination, MRI, quantum information processing and quantum computing by NMR.

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Kumar, A. Development of two-dimensional NMR. Reson 20, 995–1002 (2015). https://doi.org/10.1007/s12045-015-0267-3

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  • DOI: https://doi.org/10.1007/s12045-015-0267-3

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