Abstract
In recent years, the long-grain shape has been found as a new desired quality trait in the breeding of rice in China. The SLG7 gene is a novel gene responsible for slender grain shape in rice. So far, not much information is known regarding the functional molecular markers of SLG7. In this study, a functional marker M-SLG7 for the 11-bp deletion of promoter region of SLG7 was developed. Specificity and applicability of the marker were verified by 60 core breeding accessions and two breeding populations. The accessions, which possessed the SLG7 allele with 11-bp deletion in the promoter region, had longer grain length and better quality. Here, we recommend the use of the simple, inexpensive assay for routine genotyping of slender grain and lower chalkiness in the breeding population for discrimination of SLG7 genotype in rice germplasm.
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Acknowledgements
We thank Dr Saddam Hussain, University of Agriculture, Faisalabad-Pakistan, for critically reading and revising the manuscript. This work was funded by the National Key R&D Program in China (2018YFD0301305), the Hubei Provincial Key R&D Program (2020BBB051) and the Major R&D Program from Hubei Agricultural Science and Technology Innovation Center.
Author contributions
LZ and AY conceived and designed the experiment; LZ, EL, MC and HX performed the experiments; LZ, EL and AY analysed the data; LZ, AY and EL wrote the paper. All authors read and approved the final manuscript.
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Zhou, L., Li, E., Chang, M. et al. Development of a specific molecular marker for SLG7 on high-quality rice breeding. J Genet 100, 76 (2021). https://doi.org/10.1007/s12041-021-01330-1
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DOI: https://doi.org/10.1007/s12041-021-01330-1