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Whitefly and aphid inducible promoters of Arabidopsis thaliana L.

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Abstract

Lack of regulated expression and tissue specificity are the major drawbacks of plant and virus-derived constitutive promoters. A precise tissue or site-specific expression, facilitate regulated expression of proteins at the targeted time and site. Publically available microarray data on whitefly and aphid infested Arabidopsis thaliana L. was used to identify whitefly and aphid-inducible genes. The qRT-PCR further validated the inducible behaviour of these genes under artificial infestation. Promoter sequences of genes were retrieved from the Arabidopsis Information Resources database with their corresponding \(5^\prime \hbox {UTR}\) and cloned from the A. thaliana genome. Promoter reporter transcriptional fusions were developed with the beta-glucuronidase (GUS) gusA gene in a binary expression vector to validate the inducible behaviour of these promoters in eight independent transgenic Nicotiana tabaccum lines. Histochemical analysis of the reporter gene in \(\hbox {T}_{2}\) transgenic tobacco lines confirmed promoter driven expression at the sites of aphid and whitefly infestation. The qRT-PCR and GUS expression analysis of transgenic lines revealed that abscisic acid largely influenced the expression of both aphid and whitefly inducible promoters. Further, whitefly-specific promoter respond to salicylic acid and jasmonic acid (JA), whereas aphid-specific promoters to JA and 1-aminocyclopropane carboxylic acid. The response of promoters to phytohormones correlated to the presence of corresponding conserved cis-regulatory elements.

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Acknowledgements

NKD, DKM and DN are grateful to the Council of Scientific and Industrial Research (CSIR), India, for providing the research fellowship. This work was supported under the Council of Scientific and Industrial Research Network Project (NWP03).

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  1. CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India

    Neeraj Kumar Dubey, Devesh Kumar Mishra, Asif Idris, Deepti Nigam, Pradhyumna Kumar Singh & Samir V. Sawant

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  1. Neeraj Kumar Dubey
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  2. Devesh Kumar Mishra
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  3. Asif Idris
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  4. Deepti Nigam
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  5. Pradhyumna Kumar Singh
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  6. Samir V. Sawant
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Correspondence to Samir V. Sawant.

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Corresponding editor: Arun Joshi

NKD performed qRT-PCR, promoter cloning and transgenic analysis, and drafted this article. DN performed microarray data analysis. DKM and AI helped in transgenic development and lab experiments. PKS revised the manuscript. SVS conceived the study, participated in its design and coordination of work, data analysis and interpretation, and revised the article.

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Dubey, N.K., Mishra, D.K., Idris, A. et al. Whitefly and aphid inducible promoters of Arabidopsis thaliana L.. J Genet 97, 109–119 (2018). https://doi.org/10.1007/s12041-018-0887-y

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