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Development of molecular map and identification of QTLs linked to Fusarium wilt resistance in chickpea

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Abstract

A number of genetic maps for Fusarium wilt resistance in chickpea have been reported in earlier studies, however QTLs identified for Fusarium wilt resistance were unstable. Hence, the present study aims to map novel molecular markers and to identify QTLs for Fusarium wilt resistance in chickpea. An intraspecific linkage map of chickpea (Cicer arietinum L.) was constructed using F 10–F 11 recombinant inbred lines (RILs) derived from a cross between K850 and WR315 segregating for H 2 locus. A set of 31 polymorphic simple sequence repeat (SSR) markers obtained by screening 300 SSRs and were used for genotyping. The linkage map had four linkage groups and coverage of 690 cM with a marker density of 5.72 cM. The RILs were screened for their wilt reaction across two seasons in wilt sick plot at International Crop Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India. Five major quantitative trait loci (QTLs) were detected in both seasons for late wilting (60 days after sowing). A stable QTL (GSSR 18-TC14801) for wilt resistance was identified in both the seasons, and the QTL explained a variance of 69.80 and 60.80% in 2007 and 2008 rabi respectively.

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Acknowledgements

We thank Dr Mukesh Jain, National Institute of Plant Genome Research, New Delhi, for providing molecular markers used in the present study and the Department of Science and Technology (DST), New Delhi, for the financial support (GrantPS-170/2010).

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  1. Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India

    PAVANKUMAR JINGADE & R. L. RAVIKUMAR

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  1. PAVANKUMAR JINGADE
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  2. R. L. RAVIKUMAR
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Correspondence to PAVANKUMAR JINGADE.

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[Jingade P. and Ravikumar R. L. 2015 Development of molecular map and identification of QTLs linked to Fusarium wilt resistance in chickpea. J. Genet. 94, xx–xx]

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JINGADE, P., RAVIKUMAR, R.L. Development of molecular map and identification of QTLs linked to Fusarium wilt resistance in chickpea. J Genet 94, 723–729 (2015). https://doi.org/10.1007/s12041-015-0589-7

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