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Photochemical and photocytotoxic evaluation of new Oxovanadium (IV) complexes in photodynamic application

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Abstract

Four new ternary Oxovanadium(IV) complexes, [VO(L)(B1-4)](acac), where, L = (E)-2- (2-hydroxybenzylideneamino)-5-guanidinopentanoic acid (Argininesalisylidene) and B were 1H-imidazo-[4,5-f][1,10]-phenanthroline (B1) (1), 2-phenyl-1H-imidazo [4,5-f][1,10] phenanthroline (B2) (2), 2-(naphthalen-1-yl)-1H-imidazo [4,5-f][1,10] phenanthroline (B3) (3) or 1-(pyren-2-yl)-1H-imidazo[4,5-f][1,10] phenanthroline (B4) (4) were synthesized, characterized and photocytotoxicity in A549 cell were studied in dark and visible light (400–700 nm, 10 J cm−2). Photochemistry of the complexes (1-4) related to the generation of singlet oxygen (1O2) was studied in detail. The presence of triplet excited state in the complexes was probed from perylene assay. Photo-activated generation of singlet oxygen from the complexes was probed by DPBF assay and the degree of photo-cytotoxicity of the complexes was related to their ability in photo-activated 1O2 generation. Complexes 4 exhibited remarkable photocytotoxicity in A549 cells with photo index (PI) ~ 16 in visible light (400–700 nm, 10 J cm−2) (IC50, 3.81 µM), while complexes were almost less-toxic in dark (IC50, 59.7 µM). Intracellular ROS generation was studied from 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Annexin V-FITC/PI assay suggested apoptotic nature of cell death. Overall, the remarkable photocytotoxic efficacy of the new Oxovanadium(IV) complexes (1-4) making them the potential photochemotherapeutic agents in lieu of porphyrin-based PDT agents.

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Acknowledgements

We gratefully thank the Board of Research in Nuclear Science (BRNS), Mumbai (37(2)/14/18/2017-BRNS) for funding. We also would like to thank the National Institute of Technology, Manipur, for providing a research facility to carry out the work. We sincerely thank Prof. Akhil R Chakravarty, IISc Bangalore for providing support to carry out the project. We gratefully thank the Central Instruments Facility, IIT Guwahati for providing the EPR data.

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  1. Department of Chemistry, National Institute of Technology, Manipur, Langol, 795004, Imphal, Manipur, India

    Bandana Sanasam, Dulal Musib & Mithun Roy

  2. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, 560012, India

    Md Kausar Raza

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  1. Bandana Sanasam
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Sanasam, B., Raza, M.K., Musib, D. et al. Photochemical and photocytotoxic evaluation of new Oxovanadium (IV) complexes in photodynamic application. J Chem Sci 133, 42 (2021). https://doi.org/10.1007/s12039-021-01896-4

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