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Characterization of prolycopene-accumulated Tan406 mutant of Solanum lycopersicum

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Abstract

Significant progress has been made in understanding carotenoid biosynthesis in tomato (Solanum lycopersicum), and most pathway genes have been cloned and characterized. However, isolation and characterization of novel fruit ripening mutants is a continuous and essential process. This study describes the characterization of the Tan406 (Tangerine406) mutant of Solanum lycopersicum. Fruits of Tan406-mutant plants have a unique orange color and accumulate prolycopene instead of lycopene. Genetic analysis revealed that a monogenic recessive mutation affects fruit pigmentation in the mutant, which inhibits the conversion of prolycopene to lycopene. Further, molecular analysis indicates that fruit phenotype is attributed to loss of CRTISO gene function, which encodes a carotenoid isomerase enzyme that converts prolycopene to lycopene. The loss of gene function is due to the deletion of 406 bp from the CRTISO promoter region. Analysis of genome-wide transcriptome expression profiling identified several hundreds of differentially expressed genes in the fruit ripening stages. The results of microarray studies showed a tendency for upregulation of the genes at the mature green stage and downregulation at the fully ripened stage in the mutant. The isolated mutant can be used for the development of varieties having altered nutritional value.

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Acknowledgements

This research work was supported by University Grant Commission (UGC), New Delhi, India (F. No. 42-46/2013).

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Authors and Affiliations

  1. Department of Genetics and Genomics, School of Life Sciences, Yogi Vemana University, YSR Kadapa, Andhra Pradesh, India

    Pottipadu John Elia Prashanth, Nambi Rajesh, Mulugu Apurva Nandini, Jinka Siva Kumar, Bosetty Anjana & Osman Basha Pinjari

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  1. Pottipadu John Elia Prashanth
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  2. Nambi Rajesh
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  3. Mulugu Apurva Nandini
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  4. Jinka Siva Kumar
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  5. Bosetty Anjana
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  6. Osman Basha Pinjari
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Contributions

Conceptualization and designing of the research work, OBP; Execution of field/lab experiments and data collection, JEP, NR and JSK; analysis of data and interpretation, JEP, NR, MAN and BA; preparation of manuscript, MAN, NR and OBP.

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Correspondence to Osman Basha Pinjari.

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All authors unanimously declare that we do not have any competing interest in this regard.

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Corresponding editor: Manoj Prasad

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Prashanth, P.J.E., Rajesh, N., Nandini, M.A. et al. Characterization of prolycopene-accumulated Tan406 mutant of Solanum lycopersicum. J Biosci 48, 52 (2023). https://doi.org/10.1007/s12038-023-00389-5

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  • DOI: https://doi.org/10.1007/s12038-023-00389-5

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