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Ibuprofen Protects from Cypermethrin-Induced Changes in the Striatal Dendritic Length and Spine Density

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Abstract

Microgliosis and inflammation are major wrongdoers in cypermethrin-induced Parkinsonism along with oxidative stress, mitochondrial dysfunction and α-synuclein aggregation. Dopamine depletion could alter dendritic morphology, length and spine number in the striatum. Present study investigated the effect of ibuprofen on the dendritic morphology, length and spine density in cypermethrin PD model. Male pups were treated intraperitoneally with cypermethrin during postnatal days followed by adulthood to induce Parkinsonism using standard procedure along with controls. Subsets of animals were pre-treated with ibuprofen 2 h prior to cypermethrin treatment during adulthood. Standard methods were used to confirm Parkinsonism/neuroprotection. Striatal dendritic morphology, length, spine number and expression of synaptophysin and postsynaptic density protein-95 (PSD-95) along with the nigrostriatal pro-inflammatory and apoptotic proteins were measured. Cypermethrin induced Parkinsonian traits and attenuated the dendritic length, spine number and expression of synaptophysin and PSD-95. While cypermethrin increased the expression of interleukin-1β, interleukin-4, interferon-γ, inducible nitric oxide synthase, caspase-3, caspase-9 and B-cell lymphoma (Bcl)-xl proteins, it attenuated Bcl-2 expression. Ibuprofen normalized the changes in dendritic morphology, length, spine number and expression of synaptophysin, PSD-95, and pro-inflammatory and apoptotic proteins. Results demonstrate that cypermethrin induces inflammation and alters dendritic morphology, length and spine number, which are encountered by ibuprofen.

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Acknowledgements

Authors recognize the Council of Scientific and Industrial Research (CSIR), India for the financial support to the study (CSIR Network Project: BSC0115/miND). Indian Council of Medical Research, India, is gratefully acknowledged for extending the fellowship to Pratibha Tripathi and Ashish Singh. The CSIR-IITR communication number of this article is 3450.

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Authors and Affiliations

  1. Toxicogenomics and Predictive Toxicology Laboratory, Systems Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow, 226 001, Uttar Pradesh, India

    Pratibha Tripathi, Ashish Singh & Mahendra Pratap Singh

  2. Department of Biochemistry, Babu Banarasi Das (BBD) College of Dental Sciences, BBD University, Lucknow, 227 015, Uttar Pradesh, India

    Lakshmi Bala

  3. Academy of Scientific and Innovative Research, CSIR-IITR Campus, Lucknow, 226 001, Uttar Pradesh, India

    Devendra Kumar Patel & Mahendra Pratap Singh

  4. Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow, 226 001, Uttar Pradesh, India

    Devendra Kumar Patel

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  1. Pratibha Tripathi
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  2. Ashish Singh
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Correspondence to Mahendra Pratap Singh.

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Tripathi, P., Singh, A., Bala, L. et al. Ibuprofen Protects from Cypermethrin-Induced Changes in the Striatal Dendritic Length and Spine Density. Mol Neurobiol 55, 2333–2339 (2018). https://doi.org/10.1007/s12035-017-0491-9

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  • DOI: https://doi.org/10.1007/s12035-017-0491-9

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