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Resveratrol Represses Pokemon Expression in Human Glioma Cells

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An Erratum to this article was published on 04 June 2015

Abstract

POK erythroid myeloid ontogenic factor (Pokemon), an important proto-oncoprotein, is a transcriptional repressor that regulates the expression of many genes and plays an important role in tumorigenesis. Resveratrol (RSV), a natural polyphenolic compound, has many beneficial biological effects on health. In this study, we investigated the role of Pokemon in RSV-induced biological effects and the effect of RSV on the expression of Pokemon in glioma cells. We found that overexpression of Pokemon decreased RSV-induced cell apoptosis, senescence, and anti-proliferative effects. Moreover, we showed that RSV could efficiently decrease the activity of the Pokemon promoter and the expression of Pokemon. Meanwhile, RSV also inhibited Sp1 DNA binding activity to the Pokemon promoter; whereas, it did not influence the expression and nuclear translocation of Sp1. In addition, we found that RSV could increase the recruitment of HDAC1, but decreased p300 to the Pokemon promoter. Taken together, all these results extended our understanding on the anti-cancer mechanism of RSV in glioma cells.

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Acknowledgments

This work was supported by the grant from the National Natural Science Foundation of China (31271154, 31171032),National Basic Research Program (2010CB912003), the Development Project of Science and Technology sponsored by Beijing Education Committee (KM201310025001), and the Specialized Research Fund for the Doctoral Program of Higher Education (20111107120011).

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Authors and Affiliations

  1. Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China

    Yutao Yang, Hui Li & Zhi-Qing David Xu

  2. Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA

    Jiajun Cui & Anne-Marie Overstreet

  3. Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China

    Feng Xue

  4. Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, 100093, China

    Yiping Zhan & Chunjiang Yu

  5. Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Yiping Zhan

  6. Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China

    Dapeng Shan

  7. Department of Molecular and Biomedical Pharmacology, University of Kentucky College of Medicine, Lexington, KY, 40536, USA

    Hui Li

  8. Tianjin Anding Hospital, Tianjin, 300222, China

    Yongjun Wang

  9. Jiaxing Entry-Exit Inspection and Quarantine, Jiaxing, 314001, China

    Mengmeng Zhang

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  1. Yutao Yang
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Yang, Y., Cui, J., Xue, F. et al. Resveratrol Represses Pokemon Expression in Human Glioma Cells. Mol Neurobiol 53, 1266–1278 (2016). https://doi.org/10.1007/s12035-014-9081-2

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