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Combination Treatment with Resveratrol and Sulforaphane Induces Apoptosis in Human U251 Glioma Cells

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Abstract

Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.

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Acknowledgment

The study is supported in part by NIH grant R21 AT003463-01A2.

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Authors and Affiliations

  1. Department of Neurology, Henry Ford Hospital, Detroit, MI, 48202, USA

    Hao Jiang, Xia Shang, Grace Huang, Yiyang Wang, Shaza Al-Holou & Michael Chopp

  2. Department of Neurosurgery, Henry Ford Hospital, Detroit, MI, 48202, USA

    Hongtao Wu

  3. Department of Surgery, Henry Ford Hospital, Detroit, MI, 48202, USA

    Subhash C. Gautam

  4. Department of Physics, Oakland University, Rochester Hills, MI, 48309, USA

    Michael Chopp

Authors
  1. Hao Jiang
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  2. Xia Shang
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  4. Grace Huang
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  7. Subhash C. Gautam
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Correspondence to Hao Jiang.

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Jiang, H., Shang, X., Wu, H. et al. Combination Treatment with Resveratrol and Sulforaphane Induces Apoptosis in Human U251 Glioma Cells. Neurochem Res 35, 152–161 (2010). https://doi.org/10.1007/s11064-009-0040-7

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  • DOI: https://doi.org/10.1007/s11064-009-0040-7

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