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Screening of soil fungi in order to biosynthesize AgNPs and evaluation of antibacterial and antibiofilm activities

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Abstract

The biosynthesis of nanoparticles (NPs) has recently attracted a lot of research attention due to its being an eco-friendly and economical method. NPs are formed under normal temperatures and pressures. The shape and size of NPs can be controlled by choosing a suitable pH and temperature. In this study, 24 strains of fungi isolated from desert soils were screened for AgNP synthesis. The MS17 isolated was chosen as the superior strain capable of rapidly synthesizing monodisperse AgNPs. The optimum conditions for AgNP synthesis were investigated. AgNPs were characterized by UV–visible spectrophotometry, dynamic light scattering, X-ray diffraction, transmission electron microscopy and Fourier-transform infrared. The NPs produced were found to be in the form of Ag/AgCl with a size range of 5–15 nm. Then, the NPs were capped by proteins and carbohydrates, which play an important role in NP stability. The NPs were capable of antimicrobial activities against the standard bacterial pathogens, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 1431 and the multidrug-resistant P. aeruginosa B52 and P. aeruginosa 48.

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References

  1. Klasen H J 2000 Burns 26 131

    Article  CAS  Google Scholar 

  2. Castellano J J, Shafii S M, Ko F, Donate G, Wright T E, Mannari R J et al 2007 Int. Wound J. 4 114

    Article  Google Scholar 

  3. Klaus T, Joerger R, Olsson E and Granqvist C G 1999 Proc. Natl. Acad. Sci. 96 13614

    Article  Google Scholar 

  4. Mallick K, Witcomb M J and Scurrell M S 2004 J. Mater. Sci. 39 4459

    Article  CAS  Google Scholar 

  5. Thakkar K N, Mhatre S S and Parikh R Y 2010 Biol. Med. 6 257

    CAS  Google Scholar 

  6. Jain N, Bhargava A, Majomdar S, Tarafdar J C and Panwar J 2011 Nanoscale 3 635

    Article  CAS  Google Scholar 

  7. Sastry M, Ahmad A, Khan M I and Kumar R 2003 Curr. Sci. 85 162

    CAS  Google Scholar 

  8. Birla S S, Gaikwad S C, Gade A K and Rai M K 2013 Sci. World J. 2013 1

  9. Albalasmeh A A, Berhe A A and Ghezzehei T A 2013 Carbohydr. Polym. 97 253

    Article  CAS  Google Scholar 

  10. Bradford M M 1974 Anal. Biochem. 72 248

    Article  Google Scholar 

  11. Starcher B 2001 Anal. Biochem. 129 125

    Article  Google Scholar 

  12. Jaidev L R and Narasimha G 2010 Colloids Surf. B 81 430

    Article  CAS  Google Scholar 

  13. Maizura M, Aminah A and Wan Aida W M 2011 Int. Food Res. J. 18 529

    CAS  Google Scholar 

  14. Laemmli U K 1970 Nature 227 680

    Article  CAS  Google Scholar 

  15. CLSI 2012 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—CLSI document M07-A9 (Wayne, PA: Clinical and Laboratory Standards Institute, 9th edn.)

  16. Balakumaran M D 2016 Microbiol. Res. 182 8

    Article  CAS  Google Scholar 

  17. Sterflinger K, Tesei D and Zakharova K 2012 Fungal Ecol. 5 453

    Article  Google Scholar 

  18. Slistan-Grijalvaa A, Herrera-Urbinab R, Rivas-Silvac J F, Ávalos-Borjad M, Castillón-Barrazad F F and Posada-Amarillase A 2005 Physica E 27 104

    Article  Google Scholar 

  19. Maccuspie R I, Allen A J and Hackley V A 2011 Nanonotoxicology 5 140

    Article  CAS  Google Scholar 

  20. Mishra A, Tripathy S K, Wahab R, Jeong S H, Hwang I, Yang Y B et al 2011 Appl. Microbiol. Biotechnol. 92 617

    Article  CAS  Google Scholar 

  21. Sahoo P, Kamal S, Kumar T, Sreedhar B, Singh A and Srivastava S 2009 Def. Sci. J. 59 447

    Article  CAS  Google Scholar 

  22. Song J Y, Jang H and Kim B S 2009 Process Biochem. 44 1133

    Article  CAS  Google Scholar 

  23. Mukherjee P, Ahmad A, Mandal D, Satyajyoti S, Sudhakar R S, Mohammad I K et al 2001 Nano Lett. 1 5174

    Article  Google Scholar 

  24. Gajbhiye M, Kesharwani J, Ingle A, Gade A and Ria M 2009 Nanomed. Nanotechnol. Biol. Med. 5 382

    Article  CAS  Google Scholar 

  25. Kumar S A, Abyaneh M K, Gosavi S W, Kulkarni S K, Pasricha R, Ahmad A et al 2007 Biotechnol. Lett. 29 439

    Article  CAS  Google Scholar 

  26. Singh R, Shedbalkar U U, Wadhwani S A and Chopade B A 2015 Appl. Microbiol. Biotechnol. 99 4579

    Article  CAS  Google Scholar 

  27. Durán N, Silveria M P, Durán M and Martinez D S T 2005 J. Nanobiotechnol. 7 1

    Google Scholar 

  28. Barapatre A, Aadil K R and Jha H 2016 Bioresour. Bioprocess. 3 8

    Article  Google Scholar 

  29. Markowska K, Grudniak M, Krawczyk K, Wrobel I and Wolska K I 2014 J. Med. Microbiol. 63 849

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by Vice Chancellor Research of Alzahra University and all experiments were carried out at Sepehr Laboratories of Alzahra Universities.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, 1993893973, Iran

    Seyede Soheila Mousavi & Parinaz Ghadam

  2. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, 1993893973, Iran

    Parisa Mohammadi

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  1. Seyede Soheila Mousavi
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  2. Parinaz Ghadam
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  3. Parisa Mohammadi
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Correspondence to Parinaz Ghadam.

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Mousavi, S.S., Ghadam, P. & Mohammadi, P. Screening of soil fungi in order to biosynthesize AgNPs and evaluation of antibacterial and antibiofilm activities. Bull Mater Sci 43, 214 (2020). https://doi.org/10.1007/s12034-020-02182-8

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  • DOI: https://doi.org/10.1007/s12034-020-02182-8

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