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Evaluation of Growth and Utilization Potential of Rhodobacter sphaeroides in Reused Medium

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Abstract

Rhodobacter sphaeroides is a metabolically versatile purple non-sulfur bacteria that can produce valuable substances. As the low-cost and high-efficiency production of valuable substances is attracting attention, the reuse of the medium is emerging as a promising strategy. Therefore, in this study, the growth of R. sphaeroides was evaluated by reusing the medium of Escherichia coli and Saccharomyces cerevisiae. As a result, in the reuse of the medium in which S. cerevisiae was cultured, sufficient growth of R. sphaeroides could be confirmed, and especially, the growth of R. sphaeroides was not inhibited under aerobic conditions. Therefore, it is considered that the strategy of reusing the medium of S. cerevisiae is sufficiently feasible. Of the organic compounds investigated, R. sphaeroides grew best in succinic acid, followed by malic acid, citric acid, acetic acid, and glucose. In addition, by comparing photopigment synthesis in the reused medium, we propose the hypothesis that succinic acid may play an important role in photopigment synthesis for the first time.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant No. 2021R1A2C2093580).

Funding

Funding was provided by National Research Foundation of Korea (NRF) (Grant No. 2021R1A2C2093580).

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Author notes

  1. Yang-Hoon Kim

    Present address: School of Biological Science, Chungbuk National University, Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk, 28644, Republic of Korea

Authors and Affiliations

  1. Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea

    Jaeyoung Yu, Ju-Yong Park & Jiho Min

  2. Clean Energy Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea

    Jiho Min

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  1. Jaeyoung Yu
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Contributions

JY: Conceptualization, data curation, formal analysis, investigation, validation, writing—original draft, writing—review and editing. JYP: Data curation, formal analysis, investigation, methodology. YHK: Conceptualization, project administration, supervision. JM: Conceptualization, funding acquisition, methodology, project administration, supervision, writing—review and editing.

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Correspondence to Yang-Hoon Kim or Jiho Min.

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Yu, J., Park, JY., Kim, YH. et al. Evaluation of Growth and Utilization Potential of Rhodobacter sphaeroides in Reused Medium. Mol Biotechnol 65, 441–445 (2023). https://doi.org/10.1007/s12033-022-00553-6

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  • DOI: https://doi.org/10.1007/s12033-022-00553-6

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