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The Immunogenicity of a Novel Chimeric Hemagglutinin-Neuraminidase-Fusion Antigen from Newcastle Disease Virus by Oral Delivery of Transgenic Canola Seeds to Chickens

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Abstract

Newcastle disease (ND) is considered as one of the most devastating infectious diseases targeting domestic birds and has considerable threat to the commercial poultry production. Two surface glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F), act as antigens in the virus structure and also play important roles in infecting host cells. In the current study, the expression of the chimeric HN-F protein in canola seeds and its immunogenicity in chickens were investigated. The HN-F gene was cloned downstream of the fatty acid elongase 1 (FAE1) promoter in the binary expression vector, pBI1400-HN-F, and introduced into rapeseed (Brassica napus L.) using Agrobacterium-mediated transformation. The amount of the HN-F glycoprotein was estimated up to 0.18% and 0.11% of the total soluble protein (TSP) in transgenic seeds and leaves of canola, respectively. Confirmatory analyses of 36 transgenic lines revealed that the HN-F gene was integrated into the genome. Subsequently, HN-F protein could be expressed and accumulated in the seed tissue. Specific pathogen-free (SPF) chickens immunized orally with recombinant HN-F showed a significant rise in specific and hemagglutination inhibition (HI) antibodies 35 to 42 days post the first administration. The results implied the potential of transgenic canola seed-based expression for oral delivery of NDV immunogenic glycoproteins.

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Acknowledgements

We gratefully acknowledge the Razi Vaccine and Serum Research Institute (RVSRI) for providing the special conditions for maintaining SPF chickens and helped perform the immunization process.

Funding

This investigation was funded partially by Grant Nos. 447 and 970517-IV-702 from the National Institute of Genetic Engineering and Biotechnology (NIGEB) and National Institute for Medical Research Development (NIMAD) Grant No. 964796.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrake- Pajoohesh Blvd. 15th Km, Tehran-Karaj Highway, P.O. Box 14965-161, Tehran, Iran

    Mohammad Javad Motamedi, Rouhollah Kazemi, Mahyat Jafari & Ali-Hatef Salmanian

  2. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Jafar Amani

  3. Agricultural Research Education and Extension Organization, Razi Vaccine and Serum Research Institute, Karaj, Iran

    Mohammad Majid Ebrahimi & Shahla Shahsavandi

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  1. Mohammad Javad Motamedi
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Correspondence to Ali-Hatef Salmanian.

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Motamedi, M.J., Ebrahimi, M.M., Shahsavandi, S. et al. The Immunogenicity of a Novel Chimeric Hemagglutinin-Neuraminidase-Fusion Antigen from Newcastle Disease Virus by Oral Delivery of Transgenic Canola Seeds to Chickens. Mol Biotechnol 62, 344–354 (2020). https://doi.org/10.1007/s12033-020-00254-y

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