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Texosome-anchored superantigen triggers apoptosis in original ovarian cancer cells

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Abstract

Texosomes, nano-endosomal vesicles, are candidates for cancer immunotherapy due to their immunostimulating properties. We designed a new structure based on texosome and staphylococcal enterotoxin B (SEB) and assessed its cytotoxic impact on an ovarian cell line. Texosomes were isolated from tumor cells, and SEB was anchored onto by protein transfer method. MTT assay and Hoechst staining were used to identify the cytotoxic and apoptotic effects of this compound on treated cells with different concentrations of texosome–SEB (TEX–SEB). Moreover, the expression rate of bcl-2, bax, bak, bcl-xl and the activity of caspase-3 and caspase-9 were investigated. Treatments of the cells with 0.5, 2.5 and 10 μg/100 μl TEX–SEB were significantly cytotoxic within 24 h (p < 0.001). Hoechst staining revealed that all tested concentrations caused apoptosis after 24 h compared with the control cells (p < 0.001). Furthermore, it was found that treatment with all examined concentrations of TEX–SEB enhanced caspase-9 activity after 24 and 48 h, while caspase-3 activity was increased upon treatment with only 0.5 and 2.5 μg/100 μl of TEX–SEB after 24 h (p < 0.001). None of the concentrations of TEX–SEB affected the expression of the cancer-promoting genes. Our construct, the TEX–SEB, is a new model being able to create cytostatic properties on cancer cells.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Hamideh Mahmoodzadeh Hosseini & Abbas Ali Imani Fooladi

  2. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Jafar Soleimanirad

  3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Elnaz Mehdizadeh Aghdam

  4. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohsen Amin

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  1. Hamideh Mahmoodzadeh Hosseini
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  2. Jafar Soleimanirad
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  5. Abbas Ali Imani Fooladi
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Correspondence to Abbas Ali Imani Fooladi.

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Mahmoodzadeh Hosseini, H., Soleimanirad, J., Mehdizadeh Aghdam, E. et al. Texosome-anchored superantigen triggers apoptosis in original ovarian cancer cells. Med Oncol 32, 409 (2015). https://doi.org/10.1007/s12032-014-0409-6

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  • DOI: https://doi.org/10.1007/s12032-014-0409-6

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