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Species identification of protected carpet pythons suitable for degraded forensic samples

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Abstract

In this paper we report on the identification of a section of mitochondrial DNA that can be used to identify the species of protected and illegally traded pythons of the genus Morelia. Successful enforcement of wildlife laws requires forensic tests that can identify the species nominated in the relevant legislation. The potentially degraded state of evidentiary samples requires that forensic investigation using molecular genetic species identification is optimized to interrogate small fragments of DNA. DNA was isolated from 35 samples of Morelia spilota from which the complete cytochrome b was sequenced. The ND6 gene was also sequenced in 32 of these samples. Additional DNA sequences were generated from 9 additional species of Morelia. The sequences were aligned by Geneious and imported into MEGA to create phylogenetic trees based on the entire complex of approximately 1,706 base pairs (bp). To mimic degraded DNA, which is usually found in forensic cases, short sub-sections of the full alignment were used to generate phylogenetic trees. The sub-sections that had the greatest DNA sequence information were in parts of the cytochrome b gene. Our results highlight that legislation is presently informed by inadequate taxonomy. We demonstrated that a 278 bp region of the cytochrome b gene recovered the topology of the phylogenetic tree found with the entire gene sequence and correctly identified species of Morelia with a high degree of confidence. The locus described in this report will assist in the successful prosecution of alleged illegal trade in python species.

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Acknowledgments

Financial assistance was provided by the Department of Justice, South Australia and the Nature Foundation SA Inc. Technical assistance and advice was provided by Shanan S. Tobe, Ine van Velzen, Terry Bertozzi, Michael Gardner, Duncan Taylor, Hannah Dridan, and Steven James. Tissues were provided by Lesley Rawlings, Ken Aplin, Dave Pearson, Greg Fyfe, Ross Sadlier, Dan Natusch, Angus Emmott, Peter Robertson, Patrick Couper, Mark Hutchinson, John Weigel, Dane Trembath, Peter Hudson, Simon Stone, Terry Mazzer, David Wilson, Grant Husband, and Steven James. The authors wish to acknowledge the support of the late Prof. Ross Vining, Director of Forensic Science SA, without whom this research would not have happened.

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Authors and Affiliations

  1. School of Biological Sciences, Flinders University, Adelaide, SA, Australia

    Sherryn Ciavaglia & Adrian Linacre

  2. Forensic Science SA, Adelaide, SA, Australia

    Sherryn Ciavaglia & Julianne Henry

  3. South Australian Museum, Adelaide, SA, Australia

    Stephen Donnellan

  4. Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA, Australia

    Stephen Donnellan

Authors
  1. Sherryn Ciavaglia
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  2. Stephen Donnellan
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  4. Adrian Linacre
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Correspondence to Sherryn Ciavaglia.

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Ciavaglia, S., Donnellan, S., Henry, J. et al. Species identification of protected carpet pythons suitable for degraded forensic samples. Forensic Sci Med Pathol 10, 295–305 (2014). https://doi.org/10.1007/s12024-014-9573-4

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