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Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia

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Abstract

Introduction and aim

Hyperprolactinaemia in pregnancy leads to mild and reversible changes in the maternal skeletal system, and medicamentous hyperprolactinemia causes more detrimental effects. We conducted an experimental study to evaluate differences between Prlr gene expression in the duodenum, vertebrae and kidneys during physiological and medicamentous hyperprolactinaemia, which could influence calcium homeostasis.

Methods

Experimental animals (18 weeks old, Wistar female rats) were divided as follows: group P (nine rats that were 3 weeks pregnant), group M (ten rats that were intramuscularly administrated sulpiride (10 mg/kg) twice daily for 3 weeks), and the control group (C, ten age-matched nulliparous rats, 18-week-old). Laboratory investigations included measurements of serum ionized calcium, phosphorus, urinary calcium and phosphorus excretion, osteocalcin (OC), serum procollagen type 1 N-terminal propeptide (P1NP), vitamin D, parathyroid hormone (PTH) and prolactin (PRL). Relative quantification of gene expression for prolactin receptors in the duodenum, vertebrae and kidneys was determined using real-time PCR.

Results

Expression of the Prlr gene was significantly higher in the duodenum (p < 0.001) and lower in vertebrae (p < 0.001) and kidneys (p < 0.01) in rats with physiological hyperprolactinaemia (PHP) than in the control group. Significantly lower Prlr expression in the duodenum was verified (p < 0.001), along with increased Prlr gene expression in vertebrae (p < 0.001) and kidneys (p < 0.01), in rats with medicamentous hyperprolactinaemia (MHP) than in the C group.

Conclusions

Downregulation of Prlr gene expression in the duodenum may explain the diminished intestinal calcium absorption in medicamentous hyperprolactinaemia. Prolactin takes calcium from the skeletal system following increased Prlr gene expression in the vertebrae to maintain calcium homeostasis, which increases the harmful effect on bone metabolism compared to that of physiological hyperprolactinaemia.

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Authors and Affiliations

  1. Medical Faculty, University of Nis, Blvd.dr Zoran Djindjic 81, 18000, Nis, Serbia

    Danijela Radojkovic, Milica Pesic, Milan Radojkovic, Dragan Dimic, Marija Vukelic Nikolic, Tatjana Jevtovic Stoimenov, Sasa Radenkovic, Milena Velojic Golubovic & Slobodan Antic

  2. Clinic of Endocrinology, Diabetes and Metabolic disorders, Clinical Center Nis, Serbia Vojislav Ilic bb, 18000, Nis, Serbia

    Danijela Radojkovic, Milica Pesic, Dragan Dimic, Sasa Radenkovic, Milena Velojic Golubovic & Slobodan Antic

  3. Surgery Clinic, Clinical Center Nis, Vojislav Ilic bb, 18000, Nis, Serbia

    Milan Radojkovic

  4. Center for Medical Biochemistry, Clinical Center, Nis, Blvd.dr Zoran Djindjic 48, 18000, Nis, Serbia

    Tatjana Radjenovic Petkovic

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  1. Danijela Radojkovic
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  2. Milica Pesic
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Correspondence to Danijela Radojkovic.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The Ethical Committee of the Medical Faculty, University Nis, Serbia approved the study.

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Radojkovic, D., Pesic, M., Radojkovic, M. et al. Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia. Endocrine 62, 681–691 (2018). https://doi.org/10.1007/s12020-018-1730-1

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