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Direct stimulation of the transcellular and paracellular calcium transport in the rat cecum by prolactin

  • Transport Physiology
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Abstract

Prolactin (PRL) is reported to stimulate calcium absorption in the rat’s small intestine. However, little is known regarding its effects on the cecum, a part of the large intestine with the highest rate of intestinal calcium transport. We demonstrated herein by quantitative real-time polymerase chain reaction and Western blot analysis that the cecum could be a target organ of PRL since cecal epithelial cells strongly expressed PRL receptors. In Ussing chamber experiments, PRL enhanced the transcellular cecal calcium absorption in a biphasic dose–response manner. PRL also increased the paracellular calcium permeability and passive calcium transport in the cecum, which could be explained by the PRL-induced decrease in transepithelial resistance and increase in cation selectivity of the cecal epithelium. PRL actions in the cecum were abolished by inhibitors of phosphoinositide 3-kinase (PI3K), protein kinase C (PKC), and RhoA-associated coiled-coil forming kinase (ROCK), but not inhibitors of gene transcription and protein biosynthesis. In conclusion, PRL directly enhanced the transcellular and paracellular calcium transport in the rat cecum through the nongenomic signaling pathways involving PI3K, PKC, and ROCK.

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Acknowledgments

This work was supported by the Thailand Research Fund (RSA5180001 to N. Charoenphandhu) and the Mahidol University Postdoctoral Fellowship Program (to L.-i. Nakkrasae).

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The authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand

    Kamonshanok Kraidith, Nateetip Krishnamra & Narattaphol Charoenphandhu

  2. Consortium for Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand

    Walailuk Jantarajit, Jarinthorn Teerapornpuntakit, La-iad Nakkrasae, Nateetip Krishnamra & Narattaphol Charoenphandhu

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  1. Kamonshanok Kraidith
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  2. Walailuk Jantarajit
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  5. Nateetip Krishnamra
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Correspondence to Narattaphol Charoenphandhu.

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Supplemental Figure S1

Transepithelial calcium flux in 800 ng/mL PRL-exposed cecal tissues under open- and short-circuit conditions. The tissue was bathed on both sides with 1.25 mmol/L Ca2+-containing solution. Numbers in parentheses represent the number of animals. **p < 0.01 compared with its respective control group (GIF 15 kb)

High resolution image file (EPS 390 kb)

Supplemental Figure S2

Mucosa (M)-to-serosa (S) and S-to-M calcium fluxes in 800 ng/mL PRL-exposed cecal tissues under short-circuit condition. The removed cecal tissue from a rat was divided into two pieces for M-to-S and S-to-M studies. Net calcium flux was calculated by subtracting S-to-M calcium flux from M-to-S calcium flux. Each tissue was bathed on both sides with 1.25 mmol/L Ca2+-containing solution. Numbers in parentheses represent the number of animals. **p < 0.01 compared with its respective control group (GIF 17 kb)

High resolution image file (EPS 383 kb)

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Kraidith, K., Jantarajit, W., Teerapornpuntakit, J. et al. Direct stimulation of the transcellular and paracellular calcium transport in the rat cecum by prolactin. Pflugers Arch - Eur J Physiol 458, 993–1005 (2009). https://doi.org/10.1007/s00424-009-0679-6

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