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Preclinical Research of Stem Cells: Challenges and Progress

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Abstract

In recent years, great breakthroughs have been made in basic research and clinical applications of stem cells in regenerative medicine and other fields, which continue to inspire people to explore the field of stem cells. With nearly unlimited self-renewal ability, stem cells can generate at least one type of highly differentiated daughter cell, which provides broad development prospects for the treatment of human organ damage and other diseases. In the field of stem cell research, related technologies for inducing or isolating stem cells are relatively mature, and a variety of stable stem cell lines have been successfully constructed. To realize the full clinical application of stem cells as soon as possible, it is more and more important to further optimize each stage of stem cell research while conforming to Current Good Manufacture Practices (cGMP) standards. Here, we synthesized recent developments in stem cell research and focus on the introduction of xenogenicity in the preclinical research process and the remaining problems of various cell bioreactors. Our goal is to promote the development of technologies for xeno-free culture and clinical expansion of stem cells through in-depth discussion of current research. This review will provide new insight into stem cell research protocols and will contribute to the creation of efficient and stable stem cell expansion systems.

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Data Availability

Not applicable, no data were generated in the study.

Abbreviations

cGMP:

Current Good Manufacture Practices

ESC:

Embryonic stem cell

iPSC:

Induced pluripotent stem cell

CFD:

Computational Fluid Dynamics

hiPSCs:

Human Induced Pluripotent Stem Cells

hESCs:

Human Embryonic Stem Cells

HA-HS:

Hyaluronic acid

UCs:

Urine-derived cells

bFGF:

Basic Fibroblast Growth Factor

miPSCs:

Mouse Induced pluripotent stem cell

HBEPs:

Human breast epithelial progenitors

DE:

Definitive endoderm

HMC:

Hollow microcarrier

MSC:

Mesenchymal Stem Cell

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Acknowledgements

Thanks to Chengdu University of Traditional Chinese Medicine for providing relevant conditions.

Funding

This research was supported by the Project of Chengdu Science and Technology Bureau (No. 2022NSFSC1397), Tianfu laboratory transfer payment project (No. 2021ZYCD012).

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  1. School of Pharmacy, School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, Chengdu, China

    Jinhu Li, Yurou Wu, Xiang Yao, Yao Tian, Xue Sun, Zibo Liu & Xun Ye

  2. Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine, Chengdu, China

    Chunjie Wu

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Conceptualization: C.W., J.L.; writing—original draft preparation: J.L., Y.W.; writing—review and editing: J.L., X.Y.; visualization: J.L., Y.T., X.S., Z.L. and X.Y. ;All authors have read and agreed to the published version of the manuscript.

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Li, J., Wu, Y., Yao, X. et al. Preclinical Research of Stem Cells: Challenges and Progress. Stem Cell Rev and Rep 19, 1676–1690 (2023). https://doi.org/10.1007/s12015-023-10528-y

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