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Chemotherapeutic and Antiproliferative Effect of Purified Protein from Marine Catfish Tachysurus Dussumieri on Human Colon Cancer Cell Line

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Abstract

The present study aimed to investigate the purified protein from the epidermal mucus of marine catfish Tachysurus dussumieri on the human colon cancer cell line. The bioactive protein was purified with the Anion exchange chromatography and the collected fractions were then tested to assess cell viability in HT 29 cells through the MTT assay. The most responding active purified protein fraction (PPF III) was characterized with the MALDI-TOF/MS it shared a similar homology and sequence with 90% of antimicrobial peptides from external secretions of amphibians. Typical morphological changes of apoptotic cells, including cell shrinkage and detachment, DNA damage, and nuclear condensation were observed after the treatment of bioactive protein. PPF III triggered ROS, increasing the LDH activity, disruption of mitochondrial membrane potential, and upregulation of Cleaved caspase 3/9, Cytochrome-c, Bax, and downregulation of Bcl-2 protein and gene expression on HT 29 cells.

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Acknowledgements

The authors (K.R. and A.C.) are thankful to University Grants Commission-University research fellowship (UGC-URF), India, for providing the meritorious fellowship. Authors (P.K) also acknowledge financial support from Abu Dhabi University’s Office of Research and Sponsored Programs.

Author contributions

K.R.: Writing- Original draft preparation, Conceptualization, Methodology. P.K.: Validation, Reviewing and Editing. A.Chinnasamy: Investigation, Supervision, Reviewing and Editing.

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Authors and Affiliations

  1. Department of Zoology, University of Madras, Life Science, Guindy Campus, Chennai, 600025, Tamilnadu, India

    Kavitha Raja & Arulvasu Chinnasamy

  2. Department of Environmental and Public Health, College of Health Sciences, Abu Dhabi University, Abu Dhabi, P.O. Box 59911, United Arab Emirates

    Parashuram Kallem

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  1. Kavitha Raja
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Raja, K., Kallem, P. & Chinnasamy, A. Chemotherapeutic and Antiproliferative Effect of Purified Protein from Marine Catfish Tachysurus Dussumieri on Human Colon Cancer Cell Line. Cell Biochem Biophys 82, 247–257 (2024). https://doi.org/10.1007/s12013-023-01209-1

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